PMID- 25863010 OWN - NLM STAT- MEDLINE DCOM- 20160223 LR - 20150502 IS - 1879-1166 (Electronic) IS - 0198-8859 (Linking) VI - 76 IP - 5 DP - 2015 May TI - Association of LRP5, TCF7L2, and GCG variants and type 2 diabetes mellitus as well as fasting plasma glucose and lipid metabolism indexes. PG - 339-43 LID - S0198-8859(15)00097-X [pii] LID - 10.1016/j.humimm.2015.03.005 [doi] AB - Recent data puts WNT signaling pathway in a pivotal role in regulating pancreas development as well as islet function, insulin production and secretion. The key effectors in the WNT signaling pathway are low-density lipoprotein receptor-related protein 5 (LRP5), transcription factor 7-like 2 (TCF7L2), and downstream-regulated glucagon (GCG). Our previous studies suggest that the WNT signaling pathway plays a significant role in risk of type 2 diabetes mellitus (T2DM) in Chinese population. The main purpose of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in LRP5, TCF7L2 and glucagon (GCG) and quantitative traits in a healthy population. We used tag SNP to screen candidate SNPs for LRP5 and GCG; for TCF7L2, used the confirmed SNP rs11196218. A total of 1842 patients with T2DM and 7777 healthy controls underwent genotyping for the SNPs. We found a significant association of rs3758644 in LRP5 and fasting plasma glucose (p=0.006), and rs11196218 in TCF7L2 and triglycerides level (p=0.004). Among the SNPs in LRP5, TCF7L2, and GCG analyzed, only rs3758644 of LRP5 and rs11196218 of TCF7L2 were significantly associated with fasting plasma glucose and triglycerides index, respectively, in a healthy population. CI - Copyright (c) 2015 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved. FAU - Wang, Jinjin AU - Wang J AD - Department of Traditional Chinese Medicine Prevention, Preventive Medicine Research Evaluation Center, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, People's Republic of China. Electronic address: wangjinjin510@163.com. FAU - Yan, Guoli AU - Yan G AD - Department of Traditional Chinese Medicine Prevention, Preventive Medicine Research Evaluation Center, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, People's Republic of China. Electronic address: 784493811@qq.com. FAU - Zhang, Jianfeng AU - Zhang J AD - Henan Armed Police Corps Hospital, Zhengzhou 450000, People's Republic of China. Electronic address: besdwujing1@163.com. FAU - Gao, Kaiping AU - Gao K AD - Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: gao_kaiping@hotmail.com. FAU - Zhang, Ming AU - Zhang M AD - Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: caroline.zhang@hotmail.com. FAU - Li, Linlin AU - Li L AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: lilinlin@zzu.edu.cn. FAU - Wang, Yan AU - Wang Y AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: wangyan870828@126.com. FAU - Wang, Qian AU - Wang Q AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: zzuwq2010@163.com. FAU - Zhai, Yujia AU - Zhai Y AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: zhaiyujiamodi@163.com. FAU - You, Haifei AU - You H AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: youhaifei1987@yahoo.com.cn. FAU - Ren, Yongcheng AU - Ren Y AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: ryc12@sina.com. FAU - Wang, Bingyuan AU - Wang B AD - Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, People's Republic of China. Electronic address: wangby95@163.com. FAU - Hu, Dongsheng AU - Hu D AD - Shenzhen University School of Medicine, Shenzhen 518060, People's Republic of China. Electronic address: dongsheng_hu@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150408 PL - United States TA - Hum Immunol JT - Human immunology JID - 8010936 RN - 0 (Blood Glucose) RN - 0 (LRP5 protein, human) RN - 0 (Low Density Lipoprotein Receptor-Related Protein-5) RN - 0 (TCF7L2 protein, human) RN - 0 (Transcription Factor 7-Like 2 Protein) RN - 0 (Triglycerides) RN - 9007-92-5 (Glucagon) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Blood Glucose/analysis MH - Case-Control Studies MH - China MH - DNA Mutational Analysis MH - Diabetes Mellitus, Type 2/*genetics/metabolism MH - Female MH - Genetic Association Studies MH - Genetic Predisposition to Disease MH - Genotype MH - Glucagon/*genetics MH - Humans MH - Lipid Metabolism/genetics MH - Low Density Lipoprotein Receptor-Related Protein-5/*genetics MH - Male MH - Middle Aged MH - Polymorphism, Single Nucleotide MH - Risk MH - Severity of Illness Index MH - Transcription Factor 7-Like 2 Protein/*genetics MH - Triglycerides/metabolism MH - Wnt Signaling Pathway/genetics MH - Young Adult OTO - NOTNLM OT - Fasting plasma glucose OT - GCG OT - LRP5 OT - TCF7L2 EDAT- 2015/04/12 06:00 MHDA- 2016/02/26 06:00 CRDT- 2015/04/12 06:00 PHST- 2014/02/08 00:00 [received] PHST- 2015/01/19 00:00 [revised] PHST- 2015/03/11 00:00 [accepted] PHST- 2015/04/12 06:00 [entrez] PHST- 2015/04/12 06:00 [pubmed] PHST- 2016/02/26 06:00 [medline] AID - S0198-8859(15)00097-X [pii] AID - 10.1016/j.humimm.2015.03.005 [doi] PST - ppublish SO - Hum Immunol. 2015 May;76(5):339-43. doi: 10.1016/j.humimm.2015.03.005. Epub 2015 Apr 8.