PMID- 25863017
OWN - NLM
STAT- MEDLINE
DCOM- 20160210
LR  - 20211203
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 111
DP  - 2015
TI  - Simultaneous determination of endocannabinoids in murine plasma and brain 
      substructures by surrogate-based LC-MS/MS: Application in tumor-bearing mice.
PG  - 57-63
LID - S0731-7085(15)00194-6 [pii]
LID - 10.1016/j.jpba.2015.03.017 [doi]
AB  - The endocannabinoids (eCBs), N-arachidonoylethanolamine (anandamide, AEA) and 
      2-ararchidonylglycerol (2-AG) have been identified as main endogenous ligands for 
      cannabinoid receptors. Developing a sensitive and robust method to determine AEA 
      and 2-AG has been shown to be essential to understand their effects in stress 
      regulation and the pathogenesis of affective disorders. Since eCBs are endogenous 
      molecules, there is no true blank matrix available to construct calibration 
      curves, thus, it has been a challenge to determine eCBs in plasma and brain 
      matrix. A liquid chromatography tandem mass spectrometry (LC-MS/MS) method is 
      developed to determine the concentrations of AEA and 2-AG in murine plasma and 
      different brain substructures (prefrontal cortex, hippocampus and hypothalamus). 
      To overcome the endogenous interference, a "surrogate analyte" approach was 
      adopted using stable isotope-labeled standards as surrogates of authentic 
      analytes to generate calibration curves in biological matrix. The mobile phase, 
      composed of formic acid 0.1% in water-acetonitrile (40:60, v/v), was optimized to 
      separate 2-AG and its non-bioactive isomer 1-AG. The analytes were extracted with 
      ethyl acetate/n-hexane (9:1, v/v) and separated on an Xbridge C18 (2.1 x 100 mm, 
      3.5 mum) column using N-Oleoylethanolamine-d2 (OEA-d2) as the internal standard. 
      Detection was performed in multiple reaction monitoring (MRM) mode with an 
      electrospray ionization source operated in positive ion mode. The method was 
      applied to assess plasma and brain eCBs in tumor-bearing mice.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Gong, Yuan
AU  - Gong Y
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Li, Xinmin
AU  - Li X
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Kang, Lei
AU  - Kang L
AD  - Department of Pharmacy, Shanghai First People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Xie, Ying
AU  - Xie Y
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Rong, Zhengxing
AU  - Rong Z
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Wang, Hao
AU  - Wang H
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Qi, Hong
AU  - Qi H
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China. Electronic address: 
      qihong@shsmu.edu.cn.
FAU - Chen, Hongzhuan
AU  - Chen H
AD  - Department of Pharmacology, Institute of Medical Sciences, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150326
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Arachidonic Acids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Glycerides)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 8D239QDW64 (glyceryl 2-arachidonate)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Animals
MH  - Arachidonic Acids/blood/metabolism
MH  - Brain/*metabolism
MH  - Calibration
MH  - Chromatography, Liquid/methods
MH  - Endocannabinoids/*blood/*metabolism
MH  - Glycerides/blood/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neoplasms/*blood/*metabolism
MH  - Plasma/*chemistry
MH  - Polyunsaturated Alkamides
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - 2-AG
OT  - AEA
OT  - LC-MS/MS
OT  - Surrogate analyte
OT  - Tumor-bearing mice
EDAT- 2015/04/12 06:00
MHDA- 2016/02/11 06:00
CRDT- 2015/04/12 06:00
PHST- 2015/02/05 00:00 [received]
PHST- 2015/03/16 00:00 [revised]
PHST- 2015/03/18 00:00 [accepted]
PHST- 2015/04/12 06:00 [entrez]
PHST- 2015/04/12 06:00 [pubmed]
PHST- 2016/02/11 06:00 [medline]
AID - S0731-7085(15)00194-6 [pii]
AID - 10.1016/j.jpba.2015.03.017 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2015;111:57-63. doi: 10.1016/j.jpba.2015.03.017. Epub 2015 
      Mar 26.