PMID- 25865692
OWN - NLM
STAT- MEDLINE
DCOM- 20160707
LR  - 20191113
IS  - 1499-3872 (Print)
VI  - 14
IP  - 2
DP  - 2015 Apr
TI  - Mesenchymal stem cells from the human umbilical cord ameliorate fulminant hepatic 
      failure and increase survival in mice.
PG  - 186-93
AB  - BACKGROUND: Cell therapy has been promising for various diseases. We investigated 
      whether transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) 
      has any therapeutic effects on D-galactosamine/lipopolysaccharide 
      (GalN/LPS)-induced fulminant hepatic failure in mice. METHODS: hUCMSCs isolated 
      from human umbilical cord were cultured and transplanted via the tail vein into 
      severe combined immune deficiency mice with GalN/LPS-induced fulminant hepatic 
      failure. After transplantation, the localization and differentiation of hUCMSCs 
      in the injured livers were investigated by immunohistochemical and genetic 
      analyses. The recovery of the injured livers was evaluated histologically. The 
      survival rate of experimental animals was analyzed by the Kaplan-Meier method and 
      log-rank test. RESULTS: hUCMSCs expressed high levels of CD29, CD73, CD13, CD105 
      and CD90, but did not express CD31, CD79b, CD133, CD34, and CD45. Cultured 
      hUCMSCs displayed adipogenic and osteogenic differentiation potential. 
      Hematoxylin and eosin staining revealed that transplantation of hUCMSCs reduced 
      hepatic necrosis and promoted liver regeneration. Transplantation of hUCMSCs 
      prolonged the survival rate of mice with fulminant hepatic failure. Polymerase 
      chain reaction for human alu sequences showed the presence of human cells in 
      mouse livers. Positive staining for human albumin, human alpha-fetoprotein and 
      human cytokeratin 18 suggested the formation of hUCMSCs-derived hepatocyte-like 
      cells in vivo. CONCLUSIONS: hUCMSC was a potential candidate for stem cell based 
      therapies. After transplantation, hUCMSCs partially repaired hepatic damage 
      induced by GalN/LPS in mice. hUCMSCs engrafted into the injured liver and 
      differentiated into hepatocyte-like cells.
FAU - Yang, Jin-Feng
AU  - Yang JF
AD  - The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, 
      First Affiliated Hospital, Zhejiang University School of Medicine; Collaborative 
      Innovation Center for Diagnosis and Treatment of Infectious Diseases, 79 Qingchun 
      Road, Hangzhou 310003, China. ljli@zju.edu.cn.
FAU - Cao, Hong-Cui
AU  - Cao HC
FAU - Pan, Qiao-Ling
AU  - Pan QL
FAU - Yu, Jiong
AU  - Yu J
FAU - Li, Jun
AU  - Li J
FAU - Li, Lan-Juan
AU  - Li LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
RN  - 0 (Albumins)
RN  - 0 (Antigens, CD)
RN  - 0 (KRT18 protein, human)
RN  - 0 (Keratin-18)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (alpha-Fetoproteins)
RN  - 106441-73-0 (Osteopontin)
RN  - 7535-00-4 (Galactosamine)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
SB  - IM
MH  - Albumins/analysis
MH  - Alu Elements/genetics
MH  - Animals
MH  - Antigens, CD/*analysis
MH  - Cell Differentiation
MH  - *Cord Blood Stem Cell Transplantation
MH  - Galactosamine
MH  - Humans
MH  - Keratin-18/analysis
MH  - Lipopolysaccharides
MH  - Lipoprotein Lipase/genetics
MH  - Liver/*pathology
MH  - Liver Failure, Acute/chemically induced/pathology/*therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*chemistry
MH  - Mice
MH  - Mice, SCID
MH  - Necrosis/etiology/therapy
MH  - Osteopontin/genetics
MH  - RNA, Messenger/metabolism
MH  - Survival Rate
MH  - Tumor Necrosis Factor-alpha/blood
MH  - alpha-Fetoproteins/analysis
EDAT- 2015/04/14 06:00
MHDA- 2016/07/09 06:00
CRDT- 2015/04/14 06:00
PHST- 2015/04/14 06:00 [entrez]
PHST- 2015/04/14 06:00 [pubmed]
PHST- 2016/07/09 06:00 [medline]
AID - S1499-3872(15)60354-X [pii]
AID - 10.1016/s1499-3872(15)60354-x [doi]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2015 Apr;14(2):186-93. doi: 
      10.1016/s1499-3872(15)60354-x.