PMID- 25867039
OWN - NLM
STAT- MEDLINE
DCOM- 20160810
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 97
DP  - 2015 Mar 24
TI  - Detection of human leukocyte antigen biomarkers in breast cancer utilizing 
      label-free biosensor technology.
LID - 10.3791/52159 [doi]
LID - 52159
AB  - According to the American Cancer Society, more than 200,000 women will be 
      diagnosed with invasive breast cancer each year and approximately 40,000 will die 
      from the disease. The human leukocyte antigen (HLA) class I samples peptides 
      derived from proteasomal degradation of cellular proteins and presents these 
      fragments on the cell surface for interrogation by circulating cytotoxic T 
      lymphocytes (CTL). Generation of T-cell receptor mimic (TCRm) monoclonal 
      antibodies (mAbs) which recognize breast cancer specific peptide/HLA-A*02:01 
      complexes such as those derived from macrophage migration inhibitory factor 
      (MIF19-27) and NY-ESO-1157-165 enable detection and destruction of breast cancer 
      cells in the absence of an effective anti-tumor CTL response. Intact class I 
      HLA/peptide complexes are shed by breast cancer cells and represent potentially 
      relevant cancer biomarkers. In this work, a breakthrough biomarker screening 
      system for cancer diagnostics incorporating T-cell receptor mimic monoclonal 
      antibodies combined with a novel, label-free biosensor utilizing guided-mode 
      resonance (GMR) sensor technology is presented. Detection of shed MIF/HLA-A*02:01 
      complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma 
      is demonstrated. The impact of this work could revolutionize personalized 
      medicine through development of companion disease diagnostics for targeted 
      immunotherapies.
FAU - Weidanz, Jon A
AU  - Weidanz JA
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center.
FAU - Doll, Krysten L
AU  - Doll KL
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center.
FAU - Mohana-Sundaram, Soumya
AU  - Mohana-Sundaram S
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center.
FAU - Wichner, Timea
AU  - Wichner T
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center.
FAU - Lowe, Devin B
AU  - Lowe DB
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center.
FAU - Gimlin, Susanne
AU  - Gimlin S
AD  - Resonant Sensors Incorporated.
FAU - Wawro Weidanz, Debra
AU  - Wawro Weidanz D
AD  - Resonant Sensors Incorporated.
FAU - Magnusson, Robert
AU  - Magnusson R
AD  - Resonant Sensors Incorporated; University of Texas Arlington.
FAU - Hawkins, Oriana E
AU  - Hawkins OE
AD  - Experimmune, A Center for Immunotherapeutic Development, Department of 
      Immunotherapeutics and Biotechnology, Texas Tech University Health Sciences 
      Center; oriana.hawkins@ttuhsc.edu.
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20150324
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HLA-A*02:01 antigen)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - 0 (Peptide Fragments)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.2.1 (MIF protein, human)
SB  - IM
MH  - Antibodies, Monoclonal/chemistry/immunology
MH  - Biomarkers, Tumor/*analysis/immunology
MH  - Biosensing Techniques/*methods
MH  - Breast Neoplasms/*chemistry/immunology/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - HLA-A2 Antigen/*analysis/chemistry/immunology
MH  - Humans
MH  - Intramolecular Oxidoreductases/chemistry/immunology
MH  - Macrophage Migration-Inhibitory Factors/chemistry/immunology
MH  - Peptide Fragments/chemistry/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
PMC - PMC4401383
EDAT- 2015/04/14 06:00
MHDA- 2016/08/11 06:00
PMCR- 2015/03/24
CRDT- 2015/04/14 06:00
PHST- 2015/04/14 06:00 [entrez]
PHST- 2015/04/14 06:00 [pubmed]
PHST- 2016/08/11 06:00 [medline]
PHST- 2015/03/24 00:00 [pmc-release]
AID - 52159 [pii]
AID - 10.3791/52159 [doi]
PST - epublish
SO  - J Vis Exp. 2015 Mar 24;(97):52159. doi: 10.3791/52159.