PMID- 25868621
OWN - NLM
STAT- MEDLINE
DCOM- 20160513
LR  - 20181202
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Linking)
VI  - 53
IP  - 12
DP  - 2015
TI  - Anti-HIV-1 integrase activity and molecular docking of compounds from Albizia 
      procera bark.
PG  - 1861-6
LID - 10.3109/13880209.2015.1014568 [doi]
AB  - CONTEXT: Albizia procera (Roxb.) Benth. (Mimosaceae) has been traditionally used 
      in Thai longevity preparations. Thus, searching for HIV-1 integrase (HIV-1 IN) 
      agents from natural sources is of interest. OBJECTIVE: The objective of this 
      study is to examine the inhibitory activity against HIV-1 IN of compounds 
      isolated from the stem bark of Albizia procera. MATERIALS AND METHODS: The EtOH 
      extract and isolated compounds of Albizia procera bark were examined for 
      anti-HIV-1 IN activity at various concentrations (10-100 microg/mL and 10-100 microM) 
      using the multiplate integration assay and molecular docking. RESULTS AND 
      DISCUSSIONS: The results showed that the ethanol extract had good anti-HIV-1 IN 
      activity with an IC50 value of 19.5 microg/mL, whereas ethyl acetate fraction 
      exhibited the most potent with an IC50 value of 19.1 microg/mL, followed by water 
      fraction (IC50 value = 21.3 microg/mL), hexane and chloroform fractions (IC50 
      value > 100 microg/mL), respectively. From bioassay-guided isolation, the ethyl 
      acetate fraction was further separated to give two compounds which are 
      (+)-catechin (1) and protocatechuic acid (2), respectively. Of the tested 
      samples, (+)-catechin (1) exhibited appreciable activity against HIV-1 IN with an 
      IC50 value of 46.3 microM, whereas protocatechuic acid (2) showed mild activity with 
      46.0% inhibition at concentration of 100 microM. (+)-Catechin (1) could interact with 
      Thr66, Gly148, and Glu152 in the core domain of IN enzyme, whereas protocatechuic 
      acid (2) could bind with Thr66, His67, Glu152, Asn155, and Lys159. This is the 
      first report on anti-HIV-1 IN activity of Albizia procera bark. These results may 
      suggest that Albizia procera bark has potential as anti-HIV-1 IN agent.
FAU - Panthong, Pattarapan
AU  - Panthong P
AD  - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical 
      Sciences, Prince of Songkla University , Hat-Yai, Songkhla , Thailand .
FAU - Bunluepuech, Kingkan
AU  - Bunluepuech K
FAU - Boonnak, Nawong
AU  - Boonnak N
FAU - Chaniad, Prapaporn
AU  - Chaniad P
FAU - Pianwanit, Somsak
AU  - Pianwanit S
FAU - Wattanapiromsakul, Chatchai
AU  - Wattanapiromsakul C
FAU - Tewtrakul, Supinya
AU  - Tewtrakul S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150414
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (HIV Integrase Inhibitors)
RN  - 0 (Plant Extracts)
RN  - EC 2.7.7.- (HIV Integrase)
RN  - YY6481J2FF (p31 integrase protein, Human immunodeficiency virus 1)
SB  - IM
MH  - *Albizzia
MH  - HIV Integrase/*metabolism
MH  - HIV Integrase Inhibitors/chemistry/isolation & purification/*metabolism
MH  - Molecular Docking Simulation/*methods
MH  - *Plant Bark
MH  - Plant Extracts/chemistry/isolation & purification/*metabolism
OTO - NOTNLM
OT  - Catechin
OT  - HIV-1 IN activity
OT  - Mimosaceae
OT  - protocatechuic acid
EDAT- 2015/04/15 06:00
MHDA- 2016/05/14 06:00
CRDT- 2015/04/15 06:00
PHST- 2015/04/15 06:00 [entrez]
PHST- 2015/04/15 06:00 [pubmed]
PHST- 2016/05/14 06:00 [medline]
AID - 10.3109/13880209.2015.1014568 [doi]
PST - ppublish
SO  - Pharm Biol. 2015;53(12):1861-6. doi: 10.3109/13880209.2015.1014568. Epub 2015 Apr 
      14.