PMID- 25869072 OWN - NLM STAT- MEDLINE DCOM- 20150721 LR - 20161125 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 461 IP - 1 DP - 2015 May 22 TI - Transforming growth factor-alpha induces human ovarian cancer cell invasion by down-regulating E-cadherin in a Snail-independent manner. PG - 128-35 LID - S0006-291X(15)00648-8 [pii] LID - 10.1016/j.bbrc.2015.03.180 [doi] AB - Transforming growth factor-alpha (TGF-alpha), like epidermal growth factor (EGF) and amphiregulin (AREG) binds exclusively to EGF receptor (EGFR). We have previously demonstrated that EGF, AREG and TGF-alpha down-regulate E-cadherin and induce ovarian cancer cell invasion, though whether these ligands use the same molecular mediators remains unknown. We now show that, like EGF, TGF-alpha- and AREG-induced E-cadherin down-regulation involves both EGFR and HER2. However, in contrast to EGF and AREG, the transcription factor Snail is not required for TGF-alpha-induced E-cadherin down-regulation. This study shows that TGF-alpha uses common and divergent molecular mediators to regulate E-cadherin expression and cell invasion. CI - Copyright (c) 2015 Elsevier Inc. All rights reserved. FAU - Qiu, Xin AU - Qiu X AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - Cheng, Jung-Chien AU - Cheng JC AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - Klausen, Christian AU - Klausen C AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - Fan, Qianlan AU - Fan Q AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - Chang, Hsun-Ming AU - Chang HM AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - So, Wai-Kin AU - So WK AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. FAU - Leung, Peter C K AU - Leung PC AD - Department of Obstetrics and Gynaecology, Child & Family Research Institute, University of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada. Electronic address: peter.leung@ubc.ca. LA - eng GR - Canadian Institutes of Health Research/Canada PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150411 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 RN - 0 (AREG protein, human) RN - 0 (Amphiregulin) RN - 0 (Cadherins) RN - 0 (EGF Family of Proteins) RN - 0 (SNAI1 protein, human) RN - 0 (Snail Family Transcription Factors) RN - 0 (Transcription Factors) RN - 0 (Transforming Growth Factor beta) RN - 62229-50-9 (Epidermal Growth Factor) RN - EC 2.7.10.1 (ERBB2 protein, human) RN - EC 2.7.10.1 (Receptor, ErbB-2) SB - IM MH - Amphiregulin MH - Cadherins/*metabolism MH - Cell Line, Tumor MH - Down-Regulation MH - EGF Family of Proteins/*metabolism MH - Epidermal Growth Factor/*metabolism MH - Female MH - Humans MH - Neoplasm Invasiveness MH - Ovarian Neoplasms/*metabolism/*pathology MH - Receptor, ErbB-2/metabolism MH - Signal Transduction MH - Snail Family Transcription Factors MH - Transcription Factors/*metabolism MH - Transforming Growth Factor beta/*metabolism OTO - NOTNLM OT - E-cadherin OT - HER2 OT - Ovarian cancer OT - Slug OT - Snail OT - TGF-alpha EDAT- 2015/04/15 06:00 MHDA- 2015/07/22 06:00 CRDT- 2015/04/15 06:00 PHST- 2015/03/19 00:00 [received] PHST- 2015/03/31 00:00 [accepted] PHST- 2015/04/15 06:00 [entrez] PHST- 2015/04/15 06:00 [pubmed] PHST- 2015/07/22 06:00 [medline] AID - S0006-291X(15)00648-8 [pii] AID - 10.1016/j.bbrc.2015.03.180 [doi] PST - ppublish SO - Biochem Biophys Res Commun. 2015 May 22;461(1):128-35. doi: 10.1016/j.bbrc.2015.03.180. Epub 2015 Apr 11.