PMID- 25869278
OWN - NLM
STAT- MEDLINE
DCOM- 20160802
LR  - 20221207
IS  - 1876-2891 (Electronic)
IS  - 1876-2883 (Linking)
VI  - 6
IP  - 5
DP  - 2015
TI  - Amelioration of ovalbumin induced allergic symptoms in Balb/c mice by potentially 
      probiotic strains of lactobacilli.
PG  - 669-78
LID - 10.3920/BM2014.0141 [doi]
AB  - To evaluate the antiallergic effect of newly characterised probiotic strains, 
      Lactobacillus fermentum NWS29, Lactobacillus casei NWP08 and Lactobacillus 
      rhamnosus NWP13, mice were divided into six experimental groups: control, 
      ovalbumin (OVA), NWS29, NWP08, NWP13 and L. rhamnosus GG (LGG). Mice were 
      immunised and probiotics were administered via oral gavage followed by challenge 
      with OVA. After last challenge with OVA, inflammatory cells in bronchoalveolar 
      lavage fluid (BALF), recruitment of inflammatory cells in airways and 
      OVA-specific immunoglobulin E (IgE) in serum were determined by Giemsa, 
      haematoxylin and eosin (HE) staining, and ELISA, respectively. Relative mRNA 
      expression of interleukins (IL-4, IL-5, IL-10, IL-13 and IL-17), transforming 
      growth factor-beta (TGF-beta) and interferon-gamma (IFN-gamma) in lung and spleen tissue was 
      determined by real time RT-PCR. OVA-specific IgE levels, recruitment of 
      eosinophils and mRNA expressions of inflammatory cytokines were remarkably 
      increased in OVA-exposed mice compared with the control group. Administration of 
      NWS29 and NWP13 suppressed inflammatory cell infiltration in airways and BALF, 
      and level of OVA-specific IgE in serum of OVA-exposed mice. Furthermore, NWS29 
      and NWP13 also abrogated the mRNA expression of 1L-4, IL-5, IL-13 and TGF-beta in 
      mice immunised and exposed to OVA. Our findings suggest that NWS29 and NWP13 
      might be good candidates for the prevention of allergic airway inflammation.
FAU - Nawaz, M
AU  - Nawaz M
AD  - 1 Department of Microbiology, University of Veterinary and Animal Sciences, 
      Out-Fall Road Lahore, 54000 Lahore, Pakistan.
FAU - Ma, C
AU  - Ma C
AD  - 2 Center for Disease Control and Prevention of Xi'an, 710054 Xi'an, Shaanxi 
      Province, China P.R.
FAU - Basra, M A R
AU  - Basra MA
AD  - 3 Department of Chemistry, University of the Punjab, 54590 Lahore, Pakistan.
FAU - Wang, J
AU  - Wang J
AD  - 4 UCD Centre for Food Safety, School of Public Health, Physiotherapy and 
      Population Science, University College Dublin, Belfield, Dublin 4, Ireland.
FAU - Xu, J
AU  - Xu J
AD  - 5 Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an 
      Jiaotong University, 76 Yanta West Road, Xi'an, Shaanxi 710061, China P.R.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150422
PL  - Netherlands
TA  - Benef Microbes
JT  - Beneficial microbes
JID - 101507616
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cytokines/biosynthesis/genetics
MH  - Cytological Techniques
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Profiling
MH  - Histocytochemistry
MH  - Hypersensitivity/pathology/*therapy
MH  - Immunoglobulin E/blood
MH  - Immunologic Factors/*administration & dosage
MH  - Lacticaseibacillus casei/*immunology
MH  - Limosilactobacillus fermentum/*immunology
MH  - Lacticaseibacillus rhamnosus/*immunology
MH  - Lung/pathology
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/*immunology
MH  - Probiotics/*administration & dosage
MH  - Spleen/pathology
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Lactobacillus fermentum
OT  - allergic airway inflammation
OT  - eosinophils
OT  - interleukins
EDAT- 2015/04/15 06:00
MHDA- 2016/08/03 06:00
CRDT- 2015/04/15 06:00
PHST- 2015/04/15 06:00 [entrez]
PHST- 2015/04/15 06:00 [pubmed]
PHST- 2016/08/03 06:00 [medline]
AID - J6W57702660M0X77 [pii]
AID - 10.3920/BM2014.0141 [doi]
PST - ppublish
SO  - Benef Microbes. 2015;6(5):669-78. doi: 10.3920/BM2014.0141. Epub 2015 Apr 22.