PMID- 25870930
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20181113
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 8
DP  - 2015 Mar 7
TI  - Four core genotypes mouse model: localization of the Sry transgene and bioassay 
      for testicular hormone levels.
PG  - 69
LID - 10.1186/s13104-015-0986-2 [doi]
LID - 69
AB  - BACKGROUND: The "four core genotypes" (FCG) mouse model has emerged as a major 
      model testing if sex differences in phenotypes are caused by sex chromosome 
      complement (XX vs. XY) or gonadal hormones or both. The model involves deletion 
      of the testis-determining gene Sry from the Y chromosome and insertion of an Sry 
      transgene onto an autosome. It produces XX and XY mice with testes, and XX and XY 
      mice with ovaries, so that XX and XY mice with the same type of gonad can be 
      compared to assess phenotypic effects of sex chromosome complement in cells and 
      tissues. FINDINGS: We used PCR to amplify the Sry transgene and adjacent genomic 
      sequences, to resolve the location of the Sry transgene to chromosome 3 and 
      confirmed this location by fluorescence in situ hybridization (FISH) of the Sry 
      construct to metaphase chromosomes. Using quantitative PCR, we estimate that 
      12-14 copies of the transgene were inserted. The anogenital distance (AGD) of FCG 
      pups at 27-29 days after birth was not different in XX vs. XY males, or XX vs. XY 
      females, suggesting that differences between XX and XY mice with the same type of 
      gonad are not caused by difference in prenatal androgen levels. CONCLUSION: The 
      Sry transgene in FCG mice is present in multiple copies at one locus on 
      chromosome 3, which does not interrupt known genes. XX and XY mice with the same 
      type of gonad do not show evidence of different androgen levels prenatally.
FAU - Itoh, Yuichiro
AU  - Itoh Y
FAU - Mackie, Ryan
AU  - Mackie R
FAU - Kampf, Kathy
AU  - Kampf K
FAU - Domadia, Shelly
AU  - Domadia S
FAU - Brown, Judith D
AU  - Brown JD
FAU - O'Neill, Rachel
AU  - O'Neill R
FAU - Arnold, Arthur P
AU  - Arnold AP
LA  - eng
GR  - R01 NS043196/NS/NINDS NIH HHS/United States
GR  - R01 HD076125/HD/NICHD NIH HHS/United States
GR  - DK083561/DK/NIDDK NIH HHS/United States
GR  - NS043196/NS/NINDS NIH HHS/United States
GR  - R01 DK083561/DK/NIDDK NIH HHS/United States
GR  - HD076125/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150307
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Androgens)
SB  - IM
MH  - Androgens/genetics/*metabolism
MH  - Animals
MH  - *Biological Assay
MH  - Female
MH  - Gene Dosage
MH  - *Genes, sry
MH  - Genotype
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Ovary/growth & development/metabolism
MH  - Phenotype
MH  - *Sex Characteristics
MH  - Testis/growth & development/metabolism
MH  - Transgenes
MH  - X Chromosome/*chemistry
MH  - Y Chromosome/*chemistry
PMC - PMC4354741
EDAT- 2015/04/15 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/03/07
CRDT- 2015/04/15 06:00
PHST- 2014/10/24 00:00 [received]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/04/15 06:00 [entrez]
PHST- 2015/04/15 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/03/07 00:00 [pmc-release]
AID - s13104-015-0986-2 [pii]
AID - 986 [pii]
AID - 10.1186/s13104-015-0986-2 [doi]
PST - epublish
SO  - BMC Res Notes. 2015 Mar 7;8:69. doi: 10.1186/s13104-015-0986-2.