PMID- 25870967
OWN - NLM
STAT- MEDLINE
DCOM- 20160707
LR  - 20161018
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 53
IP  - 11
DP  - 2015 Oct
TI  - Value of a commercial kit for detecting anti-C1q autoantibodies and correlation 
      with immunological and clinical activity of lupus nephritis.
PG  - 1771-7
LID - 10.1515/cclm-2015-0072 [doi]
AB  - BACKGROUND: The association of anti-C1q antibodies (anti-C1q) with the renal 
      activity of lupus nephritis (LN) and the methods for their determination is still 
      a matter of debate. METHODS: In 116 serum samples of 66 patients with biopsy 
      proven LN, we aimed: 1) to compare the results of the determination of anti-C1q 
      obtained by a commercial kit with a clinically validated in-house ELISA; 2) to 
      evaluate the correlation of anti-C1q with the most important immunological and 
      clinical parameters employed in LN, i.e., antibodies to dsDNA (anti-dsDNA), C3 
      and C4 complement component, haemoglobin and haematuria. RESULTS: Good 
      correlation and agreement between the two methods (r=0.81, p<0.0001; contingency 
      coefficient=0.70, p<0.0001, respectively) were demonstrated. No differences were 
      observed between the two assays by ROC curves comparison. Anti-C1q levels were 
      significantly higher in patients with active LN [44 arbitrary units (AUs)] in 
      comparison to those with inactive LN (23 AUs, p=0.047) and significantly 
      correlated with anti-dsDNA (r=0.44, p<0.0001), complement fractions (C3: r=-0.33, 
      p=0.001; C4: r=-0.29, p=0.003), haemoglobin levels (r=-0.34, p=0.0004) and the 
      number of urinary red blood cells (r=0.26, p=0.01). CONCLUSIONS: Our results 
      suggest the validity of this commercial assay in detecting anti-C1q and confirm 
      the association of anti-C1q with renal involvement of LN and the importance of 
      introducing this parameter in the analytical panel for the evaluation of LN 
      activity.
FAU - de Liso, Federica
AU  - de Liso F
FAU - Matinato, Caterina
AU  - Matinato C
FAU - Novembrino, Cristina
AU  - Novembrino C
FAU - Radice, Antonella
AU  - Radice A
FAU - Raffiotta, Francesca
AU  - Raffiotta F
FAU - Ronchi, Mariangela
AU  - Ronchi M
FAU - Croci, Maria Daniela
AU  - Croci MD
FAU - Sinico, Renato Alberto
AU  - Sinico RA
FAU - Messa, Piergiorgio
AU  - Messa P
FAU - Torresani, Erminio
AU  - Torresani E
FAU - Moroni, Gabriella
AU  - Moroni G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Autoantibodies)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 80295-33-6 (Complement C1q)
SB  - IM
MH  - Autoantibodies/*blood/*immunology
MH  - Complement C1q/*immunology
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Lupus Nephritis/*blood/*immunology
MH  - *Reagent Kits, Diagnostic
EDAT- 2015/04/15 06:00
MHDA- 2016/07/09 06:00
CRDT- 2015/04/15 06:00
PHST- 2015/01/21 00:00 [received]
PHST- 2015/03/08 00:00 [accepted]
PHST- 2015/04/15 06:00 [entrez]
PHST- 2015/04/15 06:00 [pubmed]
PHST- 2016/07/09 06:00 [medline]
AID - /j/cclm.ahead-of-print/cclm-2015-0072/cclm-2015-0072.xml [pii]
AID - 10.1515/cclm-2015-0072 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2015 Oct;53(11):1771-7. doi: 10.1515/cclm-2015-0072.