PMID- 25871298
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20151106
IS  - 1423-0216 (Electronic)
IS  - 1021-7401 (Linking)
VI  - 22
IP  - 6
DP  - 2015
TI  - Short- but not long-term melatonin administration reduces central levels of 
      brain-derived neurotrophic factor in rats with inflammatory pain.
PG  - 358-64
LID - 10.1159/000380912 [doi]
AB  - OBJECTIVE: To evaluate the effect of short- and long-term administration of 
      melatonin on central brain-derived neurotrophic factor (BDNF) levels in rats with 
      acute and chronic inflammatory pain. METHODS: The animals were allocated to one 
      of two experiments: experiment 1 or experiment 2. In experiment 1, all animals 
      were injected with complete Freund's adjuvant (CFA) to induce inflammation and 
      were randomly allocated to receiving melatonin (60 mg/kg) or vehicle. Injections 
      were administered 1 h after CFA and once daily for 2 more days (for a total of 3 
      days of melatonin administration). In experiment 2, fifteen days after CFA 
      injection, the animals were treated with melatonin (50 mg/kg) or vehicle for 8 
      days. The animals were killed by decapitation 24 h after the last melatonin or 
      vehicle administration, and an ELISA assay was performed to detect BDNF 
      expression in the spinal cord, brainstem, and prefrontal cortex of the rats in 
      both groups. Data were analyzed using Student's t test and the results are 
      expressed as means +/- SEM. RESULTS: In the first experiment, the BDNF levels of 
      the melatonin group were reduced in the prefrontal cortex (Student's t test, p = 
      0.01) and increased in the spinal cord (Student's t test, p = 0.04). In 
      experiment 2, BDNF levels were similar in both groups for all structures 
      (Student's t test, p > 0.00 for all). A two-way ANOVA reveled a significant 
      effect of structures (p = 0.0001) but not of treatment (p > 0.05). The prefrontal 
      cortex presented higher BDNF levels than other structures 
      (ANOVA/Student-Newman-Keuls test, p = 0.0001). Considering the relationship 
      between BDNF levels in all three structures, we found an effect of central 
      nervous system structures (p = 0.01) and an interaction between treatment and 
      structures (p = 0.04). CONCLUSION: The high spinal cord BDNF levels and the low 
      prefrontal cortical BDNF levels observed in rats with acute CFA-induced 
      inflammation following short-term melatonin administration may be related to the 
      pain-modulating and neuroprotective effects of this protein. Long-term melatonin 
      administration did not alter BDNF levels in chronic inflammation.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Laste, Gabriela
AU  - Laste G
AD  - Pain Pharmacology and Neuromodulation, Animal Models Laboratory, Pharmacology 
      Department, Instituto de Cix00EA;ncias Bx00E1;sicas da Sax00FA;de, Universidade 
      Federal do Rio Grande do Sul, Porto Alegre, Brazil.
FAU - Ripoll Rozisky, Joanna
AU  - Ripoll Rozisky J
FAU - Caumo, Wolnei
AU  - Caumo W
FAU - Lucena da Silva Torres, Iraci
AU  - Lucena da Silva Torres I
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20150411
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Antioxidants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antioxidants/*therapeutic use
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Central Nervous System/drug effects/*metabolism
MH  - Chronic Disease
MH  - Disease Models, Animal
MH  - Freund's Adjuvant/toxicity
MH  - Inflammation/chemically induced/*complications
MH  - Melatonin/*therapeutic use
MH  - *Pain/drug therapy/etiology/metabolism
MH  - Rats
MH  - Time Factors
EDAT- 2015/04/15 06:00
MHDA- 2016/08/16 06:00
CRDT- 2015/04/15 06:00
PHST- 2014/05/28 00:00 [received]
PHST- 2015/02/11 00:00 [accepted]
PHST- 2015/04/15 06:00 [entrez]
PHST- 2015/04/15 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - 000380912 [pii]
AID - 10.1159/000380912 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 2015;22(6):358-64. doi: 10.1159/000380912. Epub 2015 Apr 
      11.