PMID- 25872871
OWN - NLM
STAT- MEDLINE
DCOM- 20160115
LR  - 20160513
IS  - 1937-9145 (Electronic)
IS  - 1945-0877 (Linking)
VI  - 8
IP  - 372
DP  - 2015 Apr 14
TI  - Constitutive production of c-di-GMP is associated with mutations in a variant of 
      Pseudomonas aeruginosa with altered membrane composition.
PG  - ra36
LID - 10.1126/scisignal.2005943 [doi]
AB  - Most bacteria can form multicellular communities called biofilms on biotic and 
      abiotic surfaces. This multicellular response to surface contact correlates with 
      an increased resistance to various adverse environmental conditions, including 
      those encountered during infections of the human host and exposure to 
      antimicrobial compounds. Biofilm formation occurs when freely swimming 
      (planktonic) cells encounter a surface, which stimulates the chemosensory-like, 
      surface-sensing system Wsp and leads to generation of the intracellular second 
      messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP). We identified 
      adaptive mutations in a clinical small colony variant (SCV) of Pseudomonas 
      aeruginosa and correlated their presence with self-aggregating growth behavior 
      and an enhanced capacity to form biofilms. We present evidence that a point 
      mutation in the 5' untranslated region of the accBC gene cluster, which encodes 
      components of an enzyme responsible for fatty acid biosynthesis, was responsible 
      for a stabilized mRNA structure that resulted in reduced translational efficiency 
      and an increase in the proportion of short-chain fatty acids in the plasma 
      membrane. We propose a model in which these changes in P. aeruginosa serve as a 
      signal for the Wsp system to constitutively produce increased amounts of c-di-GMP 
      and thus play a role in the regulation of adhesion-stimulated bacterial 
      responses.
CI  - Copyright (c) 2015, American Association for the Advancement of Science.
FAU - Blanka, Andrea
AU  - Blanka A
AD  - Institute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and 
      Experimental Infection Research, a joint venture of the Hannover Medical School 
      and the Helmholtz Center for Infection Research, 30625 Hannover, Germany.
FAU - Duvel, Juliane
AU  - Duvel J
AD  - Institute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and 
      Experimental Infection Research, a joint venture of the Hannover Medical School 
      and the Helmholtz Center for Infection Research, 30625 Hannover, Germany. 
      Department of Molecular Bacteriology, Helmholtz Center for Infection Research, 
      38124 Braunschweig, Germany.
FAU - Dotsch, Andreas
AU  - Dotsch A
AD  - Institute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and 
      Experimental Infection Research, a joint venture of the Hannover Medical School 
      and the Helmholtz Center for Infection Research, 30625 Hannover, Germany. 
      Department of Molecular Bacteriology, Helmholtz Center for Infection Research, 
      38124 Braunschweig, Germany.
FAU - Klinkert, Birgit
AU  - Klinkert B
AD  - Microbial Biology, Ruhr University Bochum, 44801 Bochum, Germany.
FAU - Abraham, Wolf-Rainer
AU  - Abraham WR
AD  - Department of Chemical Microbiology, Helmholtz Center for Infection Research, 
      38124 Braunschweig, Germany.
FAU - Kaever, Volkhard
AU  - Kaever V
AD  - Research Core Unit Metabolomics and Institute of Pharmacology, Hannover Medical 
      School, 30625 Hannover, Germany.
FAU - Ritter, Christiane
AU  - Ritter C
AD  - Department of Macromolecular Interactions, Helmholtz Center for Infection 
      Research, 38124 Braunschweig, Germany.
FAU - Narberhaus, Franz
AU  - Narberhaus F
AD  - Microbial Biology, Ruhr University Bochum, 44801 Bochum, Germany.
FAU - Haussler, Susanne
AU  - Haussler S
AD  - Institute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and 
      Experimental Infection Research, a joint venture of the Hannover Medical School 
      and the Helmholtz Center for Infection Research, 30625 Hannover, Germany. 
      Department of Molecular Bacteriology, Helmholtz Center for Infection Research, 
      38124 Braunschweig, Germany. susanne.haeussler@twincore.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150414
PL  - United States
TA  - Sci Signal
JT  - Science signaling
JID - 101465400
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Messenger)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Acetyl-CoA Carboxylase/genetics/metabolism
MH  - Bacterial Proteins/genetics/metabolism
MH  - Base Sequence
MH  - Biofilms
MH  - Cell Membrane/*metabolism
MH  - Cyclic GMP/*analogs & derivatives/biosynthesis
MH  - Cytosol/drug effects/metabolism
MH  - Fatty Acids/metabolism
MH  - Multigene Family/genetics
MH  - *Mutation
MH  - Nucleic Acid Conformation
MH  - Phenotype
MH  - Protein Biosynthesis/genetics
MH  - Pseudomonas aeruginosa/*genetics/*metabolism/physiology
MH  - RNA, Messenger/chemistry/genetics/metabolism
MH  - Sequence Homology, Nucleic Acid
MH  - Signal Transduction/drug effects/genetics
MH  - Sodium Chloride/pharmacology
EDAT- 2015/04/16 06:00
MHDA- 2016/01/16 06:00
CRDT- 2015/04/16 06:00
PHST- 2015/04/16 06:00 [entrez]
PHST- 2015/04/16 06:00 [pubmed]
PHST- 2016/01/16 06:00 [medline]
AID - 8/372/ra36 [pii]
AID - 10.1126/scisignal.2005943 [doi]
PST - epublish
SO  - Sci Signal. 2015 Apr 14;8(372):ra36. doi: 10.1126/scisignal.2005943.