PMID- 25873256 OWN - NLM STAT- MEDLINE DCOM- 20170213 LR - 20191114 IS - 1098-2825 (Electronic) IS - 0887-8013 (Print) IS - 0887-8013 (Linking) VI - 30 IP - 3 DP - 2016 May TI - Identification of Azole Resistance Markers in Clinical Isolates of Candida tropicalis Using cDNA-AFLP Method. PG - 266-72 LID - 10.1002/jcla.21847 [doi] AB - BACKGROUND: Global reports have highlighted the increasing prevalence of Candida tropicalis infections as well as organism(') s drug resistance. This study aimed at identifying azole resistance markers in clinical isolates of C. tropicalis, which will be a great resource for developing new drugs. METHODS: Two susceptible and resistant isolates of C. tropicalis were recovered from an epidemiological investigation of candidiasis in immunocompromised patients. C. tropicalis ATCC 750 was used as reference strain. Antifungal susceptibility to fluconazole and itraconazole was determined using Clinical and Laboratory Standards Institute (CLSI) method. Complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) technology and real-time reverse-transcriptase (RT) PCR were used for identification of potential genes involved in azole resistance of C. tropicalis clinical isolates. RESULTS: Five genes encoding the following enzymes were identified as superoxide dismutase (SOD) implicated in antioxidant defense, ornithine aminotransferase (OAT), acetyl ornithine aminotransferase (ACOAT), adenosylmethionine-8-amino-7-oxononanoate aminotransferase (DAPA AT), and 4-aminobutyrate aminotransferase (ABAT)-belonging to pyridoxal phosphate (PLP) dependent enzymes and acting in an important physiological role in many fungal-cell cycles. Real-time RT-PCR confirmed mRNA level of the aforementioned genes. CONCLUSION: Our findings showed that factors such as PLP-dependent enzymes and SOD might be implicated in drug resistance in C. tropicalis clinical isolate. Therefore, further studies are required to explore the accurate biological functions of the mentioned genes that would be helpful for effective drug development. CI - (c) 2015 Wiley Periodicals, Inc. FAU - Kanani, Ali AU - Kanani A AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Zaini, Farideh AU - Zaini F AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Kordbacheh, Parivash AU - Kordbacheh P AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Falahati, Mehraban AU - Falahati M AD - Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Rezaie, Sassan AU - Rezaie S AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Daie, Roshanak AU - Daie R AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Farahyar, Shirin AU - Farahyar S AD - Department of Medical Mycology and Parasitology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Safara, Mahin AU - Safara M AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Fateh, Roohollah AU - Fateh R AD - Department of Microbiology and Immunology, Faculty of Medicine, Qom University of Medical Sciences, Qom, Iran. FAU - Faghihloo, Ebrahim AU - Faghihloo E AD - Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Fattahi, Azam AU - Fattahi A AD - Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. FAU - Heidari, Mansour AU - Heidari M AD - Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran. LA - eng PT - Journal Article DEP - 20150414 PL - United States TA - J Clin Lab Anal JT - Journal of clinical laboratory analysis JID - 8801384 RN - 0 (Azoles) RN - 0 (DNA, Complementary) RN - 0 (Genetic Markers) SB - IM MH - Amplified Fragment Length Polymorphism Analysis/*methods MH - Azoles/*pharmacology MH - Candida tropicalis/*drug effects/genetics/*isolation & purification MH - DNA, Complementary/*genetics MH - Drug Resistance, Fungal/*drug effects MH - Electrophoresis, Polyacrylamide Gel MH - Gene Expression Regulation, Fungal/drug effects MH - Genetic Markers MH - Humans MH - Microbial Sensitivity Tests PMC - PMC6807149 OTO - NOTNLM OT - azole resistance OT - candidiasis OT - differential display OT - real-time RT-PCR EDAT- 2015/04/16 06:00 MHDA- 2017/02/14 06:00 PMCR- 2015/04/14 CRDT- 2015/04/16 06:00 PHST- 2014/09/18 00:00 [received] PHST- 2015/02/23 00:00 [accepted] PHST- 2015/04/16 06:00 [entrez] PHST- 2015/04/16 06:00 [pubmed] PHST- 2017/02/14 06:00 [medline] PHST- 2015/04/14 00:00 [pmc-release] AID - JCLA21847 [pii] AID - 10.1002/jcla.21847 [doi] PST - ppublish SO - J Clin Lab Anal. 2016 May;30(3):266-72. doi: 10.1002/jcla.21847. Epub 2015 Apr 14.