PMID- 25874763
OWN - NLM
STAT- MEDLINE
DCOM- 20160112
LR  - 20230403
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 16
IP  - 4
DP  - 2015 Apr 14
TI  - Production and evaluation of virus-like particles displaying immunogenic epitopes 
      of porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 8382-96
LID - 10.3390/ijms16048382 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is the most significant 
      infectious disease currently affecting the swine industry worldwide. Several 
      inactivated and modified live vaccines (MLV) have been developed to curb PRRSV 
      infections. However, the efficacy and safety of these vaccines are 
      unsatisfactory, and hence, there is a strong demand for the development of new 
      PRRS universal vaccines. Virus-like particle (VLP)-based vaccines are gaining 
      increasing acceptance compared to subunit vaccines, as they present the antigens 
      in a more veritable conformation and are readily recognized by the immune system. 
      Hepatitis B virus core antigen (HBcAg) has been successfully used as a carrier 
      for more than 100 viral sequences. In this study, hybrid HBcAg VLPs were 
      generated by fusion of the conserved protective epitopes of PRRSV and expressed 
      in E. coli. An optimized purification protocol was developed to obtain hybrid 
      HBcAg VLP protein from the inclusion bodies. This hybrid HBcAg VLP protein 
      self-assembled to 23-nm VLPs that were shown to block virus infection of 
      susceptible cells when tested on MARC 145 cells. Together with the safety of 
      non-infectious and non-replicable VLPs and the low cost of production through E. 
      coli fermentation, this hybrid VLP could be a promising vaccine candidate for 
      PRRS.
FAU - Murthy, Ambika Mosale Venkatesh
AU  - Murthy AM
AD  - Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 
      24061, USA. ambika3011@gmail.com.
FAU - Ni, Yanyan
AU  - Ni Y
AD  - Center for Molecular Medicine and Infectious Disease, Department of Biomedical 
      Sciences & Pathobiology, College of Veterinary Medicine, Virginia Tech, 
      Blacksburg, VA 24060, USA. nyy7@vt.edu.
FAU - Meng, Xiangjin
AU  - Meng X
AD  - Center for Molecular Medicine and Infectious Disease, Department of Biomedical 
      Sciences & Pathobiology, College of Veterinary Medicine, Virginia Tech, 
      Blacksburg, VA 24060, USA. xjmeng@vt.edu.
FAU - Zhang, Chenming
AU  - Zhang C
AD  - Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 
      24061, USA. chzhang2@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150414
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Epitopes)
RN  - 0 (Vaccines, Virus-Like Particle)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Epitopes/biosynthesis/immunology/isolation & purification
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Protein Refolding
MH  - Solubility
MH  - Swine
MH  - Vaccines, Virus-Like Particle/biosynthesis/*immunology/isolation & purification
PMC - PMC4425087
EDAT- 2015/04/16 06:00
MHDA- 2016/01/13 06:00
PMCR- 2015/04/14
CRDT- 2015/04/16 06:00
PHST- 2015/03/10 00:00 [received]
PHST- 2015/03/27 00:00 [revised]
PHST- 2015/04/01 00:00 [accepted]
PHST- 2015/04/16 06:00 [entrez]
PHST- 2015/04/16 06:00 [pubmed]
PHST- 2016/01/13 06:00 [medline]
PHST- 2015/04/14 00:00 [pmc-release]
AID - ijms16048382 [pii]
AID - ijms-16-08382 [pii]
AID - 10.3390/ijms16048382 [doi]
PST - epublish
SO  - Int J Mol Sci. 2015 Apr 14;16(4):8382-96. doi: 10.3390/ijms16048382.