PMID- 25874927
OWN - NLM
STAT- MEDLINE
DCOM- 20160323
LR  - 20181113
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Print)
IS  - 1615-7591 (Linking)
VI  - 38
IP  - 7
DP  - 2015 Jul
TI  - Design modification and optimisation of the perfusion system of a tri-axial 
      bioreactor for tissue engineering.
PG  - 1423-9
LID - 10.1007/s00449-015-1371-1 [doi]
AB  - A systematic design of experiments (DOE) approach was used to optimize the 
      perfusion process of a tri-axial bioreactor designed for translational tissue 
      engineering exploiting mechanical stimuli and mechanotransduction. Four 
      controllable design parameters affecting the perfusion process were identified in 
      a cause-effect diagram as potential improvement opportunities. A screening 
      process was used to separate out the factors that have the largest impact from 
      the insignificant ones. DOE was employed to find the settings of the platen 
      design, return tubing configuration and the elevation difference that minimise 
      the load on the pump and variation in the perfusion process and improve the 
      controllability of the perfusion pressures within the prescribed limits. DOE was 
      very effective for gaining increased knowledge of the perfusion process and 
      optimizing the process for improved functionality. It is hypothesized that the 
      optimized perfusion system will result in improved biological performance and 
      consistency.
FAU - Hussein, Husnah
AU  - Hussein H
AD  - Centre for Biological Engineering, The Wolfson School of Mechanical and 
      Manufacturing Engineering, Loughborough University, Loughborough, LE11 3TU, UK.
FAU - Williams, David J
AU  - Williams DJ
FAU - Liu, Yang
AU  - Liu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150210
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
SB  - IM
MH  - *Bioreactors
MH  - *Equipment Design
MH  - *Tissue Engineering
PMC - PMC4464371
EDAT- 2015/04/16 06:00
MHDA- 2016/03/24 06:00
PMCR- 2015/02/10
CRDT- 2015/04/16 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2015/01/28 00:00 [accepted]
PHST- 2015/04/16 06:00 [entrez]
PHST- 2015/04/16 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
PHST- 2015/02/10 00:00 [pmc-release]
AID - 1371 [pii]
AID - 10.1007/s00449-015-1371-1 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2015 Jul;38(7):1423-9. doi: 10.1007/s00449-015-1371-1. 
      Epub 2015 Feb 10.