PMID- 25876647
OWN - NLM
STAT- MEDLINE
DCOM- 20160308
LR  - 20180816
IS  - 1479-6821 (Electronic)
IS  - 1351-0088 (Linking)
VI  - 22
IP  - 3
DP  - 2015 Jun
TI  - Brain-derived neurotrophic factor regulates cell motility in human colon cancer.
PG  - 455-64
LID - 10.1530/ERC-15-0007 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a potent neurotrophic factor that has 
      been shown to affect cancer cell metastasis and migration. In the present study, 
      we investigated the mechanisms of BDNF-induced cell migration in colon cancer 
      cells. The migratory activities of two colon cancer cell lines, HCT116 and SW480, 
      were found to be increased in the presence of human BDNF. Heme oxygenase-1 (HO)-1 
      is known to be involved in the development and progression of tumors. However, 
      the molecular mechanisms that underlie HO-1 in the regulation of colon cancer 
      cell migration remain unclear. Expression of HO-1 protein and mRNA increased in 
      response to BDNF stimulation. The BDNF-induced increase in cell migration was 
      antagonized by a HO-1 inhibitor and HO-1 siRNA. Furthermore, the expression of 
      vascular endothelial growth factor (VEGF) also increased in response to BDNF 
      stimulation, as did VEGF mRNA expression and transcriptional activity. The 
      increase in BDNF-induced cancer cell migration was antagonized by a 
      VEGF-neutralizing antibody. Moreover, transfection with HO-1 siRNA effectively 
      reduced the increased VEGF expression induced by BDNF. The BDNF-induced cell 
      migration was regulated by the ERK, p38, and Akt signaling pathways. Furthermore, 
      BDNF-increased HO-1 and VEGF promoter transcriptional activity were inhibited by 
      ERK, p38, and AKT pharmacological inhibitors and dominant-negative mutants in 
      colon cancer cells. These results indicate that BDNF increases the migration of 
      colon cancer cells by regulating VEGF/HO-1 activation through the ERK, p38, and 
      PI3K/Akt signaling pathways. The results of this study may provide a relevant 
      contribution to our understanding of the molecular mechanisms by which BDNF 
      promotes colon cancer cell motility.
CI  - (c) 2015 Society for Endocrinology.
FAU - Huang, Ssu-Ming
AU  - Huang SM
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan Department of 
      Community MedicinePreventive Medicine CenterDivision of Colon and Rectal 
      SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, Hualien, 
      TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of Neural and 
      Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment of 
      Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 
      Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of Integrated 
      Medicine, China Medical University, Taichung, TaiwanComprehensive Breast Cancer 
      CenterDepartment of Cell and Tissue EngineeringChanghua Christian Hospital, 
      Nanxiao St., Changhua City, Changhua County 500, Taiwan Department of Community 
      MedicinePreventive Medicine CenterDivision of Colon and Rectal SurgeryDepartment 
      of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 
      Taichung, TaiwanSchool of MedicineTzu Chi University, Hualien, TaiwanDepartment 
      of PhysiologySchool of MedicineGraduate Institute of Neural and Cognitive 
      SciencesChina Medical University, Taichung, TaiwanDepartment of Internal 
      MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Ch
FAU - Lin, Chingju
AU  - Lin C
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan.
FAU - Lin, Hsiao-Yun
AU  - Lin HY
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan.
FAU - Chiu, Chien-Ming
AU  - Chiu CM
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan.
FAU - Fang, Chia-Wei
AU  - Fang CW
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan.
FAU - Liao, Kuan-Fu
AU  - Liao KF
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan Department of 
      Community MedicinePreventive Medicine CenterDivision of Colon and Rectal 
      SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, Hualien, 
      TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of Neural and 
      Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment of 
      Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 
      Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of Integrated 
      Medicine, China Medical University, Taichung, TaiwanComprehensive Breast Cancer 
      CenterDepartment of Cell and Tissue EngineeringChanghua Christian Hospital, 
      Nanxiao St., Changhua City, Changhua County 500, Taiwan Department of Community 
      MedicinePreventive Medicine CenterDivision of Colon and Rectal SurgeryDepartment 
      of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, 
      Taichung, TaiwanSchool of MedicineTzu Chi University, Hualien, TaiwanDepartment 
      of PhysiologySchool of MedicineGraduate Institute of Neural and Cognitive 
      SciencesChina Medical University, Taichung, TaiwanDepartment of Internal 
      MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Ch
FAU - Chen, Dar-Ren
AU  - Chen DR
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan.
FAU - Yeh, Wei-Lan
AU  - Yeh WL
AD  - Department of Community MedicinePreventive Medicine CenterDivision of Colon and 
      Rectal SurgeryDepartment of Surgery, Taichung Tzu Chi Hospital, Buddhist Tzu Chi 
      Medical Foundation, Taichung, TaiwanSchool of MedicineTzu Chi University, 
      Hualien, TaiwanDepartment of PhysiologySchool of MedicineGraduate Institute of 
      Neural and Cognitive SciencesChina Medical University, Taichung, TaiwanDepartment 
      of Internal MedicineTaichung Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Taichung, TaiwanDepartment of Chinese MedicineGraduate Institute of 
      Integrated Medicine, China Medical University, Taichung, TaiwanComprehensive 
      Breast Cancer CenterDepartment of Cell and Tissue EngineeringChanghua Christian 
      Hospital, Nanxiao St., Changhua City, Changhua County 500, Taiwan 
      ibizayeh0816@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150415
PL  - England
TA  - Endocr Relat Cancer
JT  - Endocrine-related cancer
JID - 9436481
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Brain-Derived Neurotrophic Factor/*blood/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Colonic Neoplasms/genetics/*metabolism/*pathology
MH  - Enzyme Induction
MH  - HCT116 Cells
MH  - Heme Oxygenase-1/biosynthesis/genetics
MH  - Humans
MH  - Signal Transduction
MH  - Transfection
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/biosynthesis/genetics
OTO - NOTNLM
OT  - BDNF
OT  - HO-1
OT  - VEGF
OT  - colon cancer
OT  - migration
EDAT- 2015/04/17 06:00
MHDA- 2016/03/10 06:00
CRDT- 2015/04/17 06:00
PHST- 2015/04/14 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
AID - ERC-15-0007 [pii]
AID - 10.1530/ERC-15-0007 [doi]
PST - ppublish
SO  - Endocr Relat Cancer. 2015 Jun;22(3):455-64. doi: 10.1530/ERC-15-0007. Epub 2015 
      Apr 15.