PMID- 25877819
OWN - NLM
STAT- MEDLINE
DCOM- 20150720
LR  - 20150513
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 160
IP  - 6
DP  - 2015 Jun
TI  - Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine 
      candidate onto biodegradable nanoparticles improves immunogenicity in mice.
PG  - 1543-7
LID - 10.1007/s00705-015-2396-0 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is an economically 
      devastating viral disease of pigs. Safer and more effective vaccines are urgently 
      needed. In this study, a synthetic ORF5 gene of porcine reproductive and 
      respiratory syndrome virus (PRRSV) was adsorbed onto poly(D, 
      L-lactide-co-glycolide)/polyethylenimine (PLGA/PEI) nanoparticles. We prepared a 
      PLGA-nanoparticle-adsorbed PRRSV DNA vaccine and a PEI-DNA complex. The results 
      showed that these model vaccines could significantly enhance humoral and cellular 
      immune responses when compared with the responses induced by pcDNA3.1-SynORF5, a 
      plasmid construct for expression of PRRSV ORF5. PLGA-branched PEI nanoparticles 
      induced the most efficient immune response. The delivery system and adjuvant 
      provide new models for the development of vaccines against PRRSV.
FAU - Du, Luping
AU  - Du L
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, 50 
      Zhong-ling Street, Nanjing, 210014, Jiangsu , China.
FAU - Li, Bin
AU  - Li B
FAU - Xu, Xiangwei
AU  - Xu X
FAU - Sun, Bing
AU  - Sun B
FAU - Pang, Fengjiao
AU  - Pang F
FAU - Wen, Libin
AU  - Wen L
FAU - Huang, Kehe
AU  - Huang K
FAU - He, Kongwang
AU  - He K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150401
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Vaccines, DNA)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Immunity, Cellular/immunology
MH  - Immunity, Humoral/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanoparticles/*therapeutic use
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccines, DNA/*therapeutic use
MH  - Viral Envelope Proteins/genetics/therapeutic use
EDAT- 2015/04/17 06:00
MHDA- 2015/07/21 06:00
CRDT- 2015/04/17 06:00
PHST- 2014/11/27 00:00 [received]
PHST- 2015/03/12 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2015/07/21 06:00 [medline]
AID - 10.1007/s00705-015-2396-0 [doi]
PST - ppublish
SO  - Arch Virol. 2015 Jun;160(6):1543-7. doi: 10.1007/s00705-015-2396-0. Epub 2015 Apr 
      1.