PMID- 25879046
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Print)
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 5
IP  - 4
DP  - 2015 Feb 20
TI  - Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry.
LID - e1406 [pii]
AB  - This protocol describes a straightforward technique to evaluate the phagocytotic 
      capacity of murine macrophages for Staphylococcus aureus (S. aureus). By staining 
      S. aureus with Hexidium Iodide and staining murine bone marrow-derived 
      macrophages (BMDMs) with FITC, the macrophage bacterial up-taking ability can be 
      rapidly analyzed by flow cytometry. S. aureus is a Gram-positive bacteria causing 
      severe human and animal infections. Host immune cells such as macrophages serve 
      to eliminate S. aureus by phagocytosing the pathogen and save the host from 
      life-threatening diseases. Study of host macrophage ability to phagocytose S. 
      aureus is important for understanding the host-pathogen interaction and can help 
      to elucidate the pathogenesis of S. aureus infection. This protocol may also be 
      applied for macrophage phagocytotic assay of other gram-positive bacteria.
FAU - Yan, Qin
AU  - Yan Q
AD  - Duke University Medical Center, Durham, North Carolina.
FAU - Ahn, Sun Hee
AU  - Ahn SH
AD  - Chonnam National University, Gwangju, Korea.
FAU - Fowler, Vance G Jr
AU  - Fowler VG Jr
AD  - Duke University Medical Center, Durham, North Carolina.
LA  - eng
GR  - K24 AI093969/AI/NIAID NIH HHS/United States
GR  - R01 AI068804/AI/NIAID NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC4394738
MID - NIHMS674907
EDAT- 2015/04/17 06:00
MHDA- 2015/04/17 06:01
PMCR- 2015/04/13
CRDT- 2015/04/17 06:00
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2015/04/17 06:01 [medline]
PHST- 2015/04/13 00:00 [pmc-release]
AID - e1406 [pii]
AID - 10.21769/bioprotoc.1406 [doi]
PST - ppublish
SO  - Bio Protoc. 2015 Feb 20;5(4):e1406. doi: 10.21769/bioprotoc.1406.