PMID- 25879634
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR  - 20181113
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 11
DP  - 2015 Feb 28
TI  - Rapid and specific detection of porcine parvovirus using real-time PCR and high 
      resolution melting (HRM) analysis.
PG  - 46
LID - 10.1186/s12917-015-0364-2 [doi]
LID - 46
AB  - BACKGROUND: Porcine parvovirus (PPV) is the important causative agent for 
      infectious infertility, which is a fairly tough virus that multiplies normally in 
      the intestine of pigs without causing clinical signs in the world. RESULTS: We 
      developed an assay integrating real-time PCR and high resolution melting (HRM) 
      analysis for the detection of PPV. Primers targeting the VP gene were highly 
      specific, as evidenced by the negative amplification of closely related viruses, 
      such as porcine circovirus 2 (PCV2), porcine reproductive and respiratory 
      syndrome virus (PRRSV), pseudorabies virus (PRV), classical swine fever virus 
      (CSFV), or Japanese encephalitis virus (JEV). The performance of unlabeled real 
      time PCR was compared to TaqMan real time PCR, and the detection limits of the 
      two methods were nearly equal. Moreover, there was good correlation between Cp 
      and diluted genomic DNA when tested with the two methods. The assay has the 
      accuracy of 100% in reference to labeled real time PCR, when it was tested on 45 
      clinical samples. CONCLUSIONS: The present study demonstrated that the 
      established assay integrating real-time PCR and HRM is relatively cost-effective 
      and more stable, which provides an alternative tool for rapid, simple, specific 
      and sensitive detection of PPV.
FAU - Yu, Hai-Qiong
AU  - Yu HQ
AD  - Technical Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, 
      Guangzhou, Guangdong Province, 510630, PR China. haiqiongyu@139.com.
FAU - Cai, Xian-Quan
AU  - Cai XQ
AD  - Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, 
      Zhongshan, Guangdong Province, 528403, PR China. caixianquan@126.com.
FAU - Lin, Zhi-Xiong
AU  - Lin ZX
AD  - Technical Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, 
      Guangzhou, Guangdong Province, 510630, PR China. 13922289528@139.com.
FAU - Li, Xiang-Li
AU  - Li XL
AD  - Zhongshan Torch Polytechnic College, Zhongshan, Guangdong Province, 528403, PR 
      China. cap1025@sina.com.
FAU - Yue, Qiao-Yun
AU  - Yue QY
AD  - Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, 
      Zhongshan, Guangdong Province, 528403, PR China. 914529077@qq.com.
FAU - Li, Rong
AU  - Li R
AD  - Technical Center, Zhongshan Entry-Exit Inspection and Quarantine Bureau, 
      Zhongshan, Guangdong Province, 528403, PR China. lir@zs.gdciq.gov.cn.
FAU - Zhu, Xing-Quan
AU  - Zhu XQ
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 
      Province, 730046, PR China. xingquanzhu1@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150228
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral/genetics
MH  - Kidney/virology
MH  - Limit of Detection
MH  - Liver/virology
MH  - Lung/virology
MH  - Nucleic Acid Denaturation
MH  - Parvoviridae Infections/diagnosis/*veterinary
MH  - *Parvovirus, Porcine/genetics
MH  - Real-Time Polymerase Chain Reaction/*veterinary
MH  - Sensitivity and Specificity
MH  - Spleen/virology
MH  - Swine/virology
MH  - Swine Diseases/*diagnosis/virology
PMC - PMC4356059
EDAT- 2015/04/17 06:00
MHDA- 2016/04/09 06:00
PMCR- 2015/02/28
CRDT- 2015/04/17 06:00
PHST- 2014/09/18 00:00 [received]
PHST- 2015/02/19 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
PHST- 2015/02/28 00:00 [pmc-release]
AID - 10.1186/s12917-015-0364-2 [pii]
AID - 364 [pii]
AID - 10.1186/s12917-015-0364-2 [doi]
PST - epublish
SO  - BMC Vet Res. 2015 Feb 28;11:46. doi: 10.1186/s12917-015-0364-2.