PMID- 25879828
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20181113
IS  - 1475-2875 (Electronic)
IS  - 1475-2875 (Linking)
VI  - 14
DP  - 2015 Feb 7
TI  - Mast cell activation in the skin of Plasmodium falciparum malaria patients.
PG  - 67
LID - 10.1186/s12936-015-0568-8 [doi]
LID - 67
AB  - BACKGROUND: Mast cells (MCs) play an important role in the immune response and 
      inflammatory processes. Generally, MCs can be stimulated to degranulate and 
      release histamine upon binding to immunoglobulin E (IgE). In malaria, MCs have 
      been linked to immunoglobulin (Ig) E-anti-malarial antibodies. This study 
      investigated the response of MCs in the skin of patients with Plasmodium 
      falciparum malaria. METHODS: Skin tissue samples were examined from ten 
      uncomplicated and 20 complicated P. falciparum malaria cases. Normal skin tissues 
      from 29 cases served as controls. Pre- and post-treatment tissues were included. 
      Histopathological changes of the skin were evaluated using haematoxylin and eosin 
      stain. MCs were investigated using toluidine blue staining. The percentage of MC 
      degranulation was compared among groups and correlated with clinical data. 
      RESULTS: MC degranulation was significantly higher in the complicated P. 
      falciparum (43.72% +/- 1.44) group than the uncomplicated P. falciparum 
      (31.35% +/- 3.29) (p <0.05) and control groups (18.38% +/- 1.75), (p <0.0001). MC 
      degranulation correlated significantly with the degree of parasitaemia (rs 
       = 0.66, p <0.0001). Associated pathological features, including extravasation of 
      red blood cells, perivascular oedema and leukocyte infiltration were 
      significantly increased in the malaria groups compared with the control group 
      (all p <0.001). CONCLUSIONS: MCs in the skin dermis are activated during malaria 
      infection, and the degree of MC degranulation correlates with parasitaemia and 
      disease severity.
FAU - Wilainam, Panop
AU  - Wilainam P
AD  - Department of Preclinic and Applied Animal Science, Faculty of Veterinary 
      Science, Mahidol University, Nakhonpathom, 73170, Thailand. 
      panop.wil@mahidol.ac.th.
FAU - Nintasen, Rungrat
AU  - Nintasen R
AD  - Department of Biological Science, Faculty of Science and Technology, Huachiew 
      Chalermprakiet University, 18/18 Bang Na-Trad Road, Km 18, Samut Prakarn, 10540, 
      Thailand. n_rungrat@yahoo.com.
FAU - Viriyavejakul, Parnpen
AU  - Viriyavejakul P
AD  - Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol 
      University, 420/6 Rajvithi Road, Bangkok, 10400, Thailand. 
      parnpen.vir@mahidol.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150207
PL  - England
TA  - Malar J
JT  - Malaria journal
JID - 101139802
RN  - 0 (Coloring Agents)
RN  - 15XUH0X66N (Tolonium Chloride)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Coloring Agents
MH  - Erythrocytes/parasitology
MH  - Female
MH  - Humans
MH  - Malaria, Falciparum/*immunology
MH  - Male
MH  - Mast Cells/*immunology
MH  - Skin/*immunology
MH  - Tolonium Chloride
PMC - PMC4326495
EDAT- 2015/04/17 06:00
MHDA- 2016/02/09 06:00
PMCR- 2015/02/07
CRDT- 2015/04/17 06:00
PHST- 2014/11/07 00:00 [received]
PHST- 2015/01/16 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
PHST- 2015/02/07 00:00 [pmc-release]
AID - 10.1186/s12936-015-0568-8 [pii]
AID - 568 [pii]
AID - 10.1186/s12936-015-0568-8 [doi]
PST - epublish
SO  - Malar J. 2015 Feb 7;14:67. doi: 10.1186/s12936-015-0568-8.