PMID- 25879927
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20201209
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 4
DP  - 2015
TI  - Correction: Membrane-active macromolecules resensitize NDM-1 gram-negative 
      clinical isolates to tetracycline antibiotics.
PG  - e0126757
LID - 10.1371/journal.pone.0126757 [doi]
LID - e0126757
AB  - Gram-negative 'superbugs' such as New Delhi metallo-beta-lactamase-1 (blaNDM-1) 
      producing pathogens have become world's major public health threats. Development 
      of molecular strategies that can rehabilitate the 'old antibiotics' and halt the 
      antibiotic resistance is a promising approach to target them. We report 
      membrane-active macromolecules (MAMs)that restore the antibacterial efficacy 
      (enhancement by >80-1250 fold) of tetracycline antibiotics towards blaNDM-1 
      Klebsiella pneumonia and blaNDM-1 Escherichia coli clinical isolates.Organismic 
      studies showed that bacteria had an increased and faster uptake of tetracyclinein 
      the presence of MAMs which is attributed to the mechanism of re-sensitization. 
      Moreover,bacteria did not develop resistance to MAMs and MAMs stalled the 
      development of bacterial resistance to tetracycline. MAMs displayed 
      membrane-active properties such as dissipation of membrane potential and 
      membrane-permeabilization that enabled higher uptake of tetracycline in bacteria. 
      In-vivo toxicity studies displayed good safety profiles and preliminary in-vivo 
      antibacterial efficacy studies showed that mice treated with MAMs in combination 
      with antibiotics had significantly decreased bacterial burden compared to the 
      untreated mice. This report of re-instating the efficacy of the antibiotics 
      towards blaNDM-1 pathogens using membrane-active molecules advocates their 
      potential for synergistic co-delivery of antibiotics to combat Gram-negative 
      superbugs.
FAU - Uppu, Divakara S S M
AU  - Uppu DS
FAU - Manjunath, Goutham B
AU  - Manjunath GB
FAU - Yarlagadda, Venkateswarlu
AU  - Yarlagadda V
FAU - Kaviyil, Jyothi E
AU  - Kaviyil JE
FAU - Ravikumar, Raju
AU  - Ravikumar R
FAU - Paramanandham, Krishnamoorthy
AU  - Paramanandham K
FAU - Shome, Bibek R
AU  - Shome BR
FAU - Haldar, Jayanta
AU  - Haldar J
LA  - eng
PT  - Journal Article
PT  - Published Erratum
PT  - Research Support, Non-U.S. Gov't
DEP - 20150416
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Bacterial Agents)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - EC 3.5.2.6 (beta-lactamase NDM-1)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
EFR - PLoS One. 2015;10(3):e0119422. PMID: 25789871
MH  - Animals
MH  - Anti-Bacterial Agents/administration & dosage
MH  - Cell Membrane/*metabolism
MH  - Drug Resistance, Bacterial/*drug effects
MH  - Escherichia coli/drug effects/isolation & purification
MH  - Escherichia coli Infections/*drug therapy/microbiology
MH  - Humans
MH  - Klebsiella Infections/*drug therapy/microbiology
MH  - Klebsiella pneumoniae/drug effects/isolation & purification
MH  - Mice
MH  - Tetracycline/*administration & dosage
MH  - beta-Lactamases/*metabolism
PMC - PMC4400169
EDAT- 2015/04/17 06:00
MHDA- 2016/04/20 06:00
PMCR- 2015/04/16
CRDT- 2015/04/17 06:00
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
PHST- 2015/04/16 00:00 [pmc-release]
AID - PONE-D-15-15498 [pii]
AID - 10.1371/journal.pone.0126757 [doi]
PST - epublish
SO  - PLoS One. 2015 Apr 16;10(4):e0126757. doi: 10.1371/journal.pone.0126757. 
      eCollection 2015.