PMID- 25880248
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20150505
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 85
IP  - 6
DP  - 2015 Jun
TI  - A comprehensive analysis of peptides presented by HLA-A1.
PG  - 492-6
LID - 10.1111/tan.12565 [doi]
AB  - Human leukocyte antigen (HLA)-A1 is one of the most common Caucasian HLA-A 
      alleles. Here, we describe the comprehensive analysis of the HLA-A*01:01 ligand 
      repertoire with the identification of 4735 naturally processed and presented 
      peptides derived from 2477 source proteins. We found HLA-A*01:01 bound an 
      equivalent number of ligands of 9 or 10 amino acids in length as well as being 
      remarkably tolerant of even longer peptides. Indeed close to half of the HLA-A1 
      bound peptides identified ranged between 11 and 13 amino acids in length. These 
      longer peptides contained the strong canonical motif of and acidic E/D residue at 
      position 3 (P3) and Y at the C-terminus (COmega), a motif that was still apparent in 
      peptides of up to 18 amino acids in length. The identification of this large 
      database of natural ligands will facilitate the refinement of predictive 
      algorithms particularly with respect to longer peptide ligands.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Giam, K
AU  - Giam K
AD  - Department of Biochemistry and Molecular Biology, Monash University, Clayton, 
      Victoria 3800, Australia; The Department of Biochemistry and Molecular Biology, 
      The Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 
      Parkville, Victoria 3010, Australia.
FAU - Ayala-Perez, R
AU  - Ayala-Perez R
FAU - Illing, P T
AU  - Illing PT
FAU - Schittenhelm, R B
AU  - Schittenhelm RB
FAU - Croft, N P
AU  - Croft NP
FAU - Purcell, A W
AU  - Purcell AW
FAU - Dudek, N L
AU  - Dudek NL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150416
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-A*01:01 antigen)
RN  - 0 (HLA-A1 Antigen)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - *Antigen Presentation
MH  - B-Lymphocytes/immunology
MH  - Cell Line, Transformed
MH  - HLA-A1 Antigen/*immunology
MH  - Humans
MH  - Ligands
MH  - Peptides/chemistry/*immunology
MH  - Recombinant Proteins/immunology
MH  - Transfection
OTO - NOTNLM
OT  - antigen presentation
OT  - human leukocyte antigen-A1
OT  - immunopeptidome
OT  - mass spectrometry
OT  - peptides
EDAT- 2015/04/17 06:00
MHDA- 2016/04/20 06:00
CRDT- 2015/04/17 06:00
PHST- 2014/12/09 00:00 [received]
PHST- 2015/03/16 00:00 [revised]
PHST- 2015/03/22 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
AID - 10.1111/tan.12565 [doi]
PST - ppublish
SO  - Tissue Antigens. 2015 Jun;85(6):492-6. doi: 10.1111/tan.12565. Epub 2015 Apr 16.