PMID- 25880659 OWN - NLM STAT- MEDLINE DCOM- 20151214 LR - 20240322 IS - 1472-6882 (Electronic) IS - 1472-6882 (Linking) VI - 15 DP - 2015 Apr 9 TI - Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08. PG - 111 LID - 10.1186/s12906-015-0630-7 [doi] LID - 111 AB - BACKGROUND: In the modern society, aging had been a major problem. People may rely on many medicines to delay it. However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body. Microbial sources could be used as a potential means of producing natural antioxidants. Lentinus edodes, commercial obtained in daily life, had recently become more attractive in physiological research. Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks. To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed. METHODS: Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately. The zinc content was determined by flame atomic absorption spectrometry. The evaluation of monosaccharide compositions and proportions used gas chromatogram. The analysis of molecular weight used HPGPC chromatogram. The typical structure of polysaccharide was evaluated by IR spectrum. The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). RESULTS: MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained. In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography. Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS. MPS also had the typical absorption of -NH3 (+), -NH2 and -COOH. Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA). CONCLUSIONS: MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant. FAU - Wang, Liqin AU - Wang L AD - College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. diandianmeng@126.com. FAU - Wang, Cuiqin AU - Wang C AD - Government Hospital of Yantai, Yuxi Road 16, Yantai, Shandong, 264000, PR China. wangcuiqin@163.com. FAU - Gao, Xia AU - Gao X AD - Shandong Agricultural Technology Extending Station, Jinan, Shandong, 250100, PR China. gaoxia@163.com. FAU - Xu, Nuo AU - Xu N AD - College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. xunuo@163.com. FAU - Lin, Lin AU - Lin L AD - College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. linlin@163.com. FAU - Zhao, Huajie AU - Zhao H AD - College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. zhaohuajie@163.com. FAU - Jia, Shouhua AU - Jia S AD - College of Chemistry and Material Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. shjia@sdau.edu.cn. FAU - Jia, Le AU - Jia L AD - College of Life Science, Shandong Agricultural University, Daizong Street 61, Taian, Shandong, 271018, PR China. ourlaboratory@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150409 PL - England TA - BMC Complement Altern Med JT - BMC complementary and alternative medicine JID - 101088661 RN - 0 (Antioxidants) RN - 0 (Biological Products) RN - 0 (Biphenyl Compounds) RN - 0 (Picrates) RN - 0 (Polysaccharides) RN - 4Y8F71G49Q (Malondialdehyde) RN - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - J41CSQ7QDS (Zinc) SB - IM MH - Aging/*drug effects/metabolism MH - Animals MH - Antioxidants/metabolism/*pharmacology MH - Biological Products/*pharmacology MH - Biphenyl Compounds/metabolism MH - Humans MH - Male MH - Malondialdehyde/blood MH - Mice MH - Mycelium/metabolism MH - Oxidation-Reduction MH - Oxidative Stress/*drug effects MH - Picrates/metabolism MH - Polysaccharides/chemistry/*pharmacology MH - Shiitake Mushrooms/*metabolism MH - Superoxide Dismutase/metabolism MH - Zinc/*pharmacology PMC - PMC4409736 EDAT- 2015/04/17 06:00 MHDA- 2015/12/15 06:00 PMCR- 2015/04/09 CRDT- 2015/04/17 06:00 PHST- 2014/11/19 00:00 [received] PHST- 2015/03/23 00:00 [accepted] PHST- 2015/04/17 06:00 [entrez] PHST- 2015/04/17 06:00 [pubmed] PHST- 2015/12/15 06:00 [medline] PHST- 2015/04/09 00:00 [pmc-release] AID - 10.1186/s12906-015-0630-7 [pii] AID - 630 [pii] AID - 10.1186/s12906-015-0630-7 [doi] PST - epublish SO - BMC Complement Altern Med. 2015 Apr 9;15:111. doi: 10.1186/s12906-015-0630-7.