PMID- 25880834
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20201223
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 71
DP  - 2015 Sep 15
TI  - Self-powered competitive immunosensor driven by biofuel cell based on 
      hollow-channel paper analytical devices.
PG  - 18-24
LID - S0956-5663(15)30020-8 [pii]
LID - 10.1016/j.bios.2015.04.019 [doi]
AB  - A mediator-less and compartment-less glucose/O2 enzymatic biofuel cell (BFC) was 
      introduced into microfluidic paper-based analytical devices (mu-PADs) that relies 
      on flow in hollow channels with silver nanoparticles/graphene modified paper 
      electrode as the anodic and cathodic substrate, to implement self-powered 
      sensitive carcinoembryonic antigen (CEA) detection. Glucose dehydrogenase 
      (GDH)-gold nanoparticles bioconjugate modified with CEA acted as a biocatalyst 
      for enhancing glucose oxidation in the bioanode, as well as the transducing 
      enzyme for signaling magnification. Similarly, nanoporous PtNi/bilirubin oxidase 
      (BOD) acted as a biocatalyst for enhancing O2 reduction in the biocathode. With 
      an increase in the concentration of CEA, the amount of CEA-Au-GDH bioconjugate on 
      bioanode decreases, thus leading to the lower output of the as-prepared BFC. This 
      proposed BFC-based self-powered immunosensor for CEA possessed largely increased 
      linear detection range from 1 pg mL(-1) to 0.5 mug mL(-)(1) with a detection limit 
      of 0.7 pg mL(-)(1). The proposed BFC-based self-powered immunosensor shows high 
      sensitivity, stability, and reproducibility and can become a promising platform 
      for other protein detection.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Li, Shuai
AU  - Li S
AD  - Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School 
      of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR 
      China.
FAU - Wang, Yanhu
AU  - Wang Y
AD  - Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School 
      of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR 
      China.
FAU - Ge, Shenguang
AU  - Ge S
AD  - Shandong Provincial Key Laboratory of Preparation and Measurement of Building 
      Materials, School of Material Science and Engineering, University of Jinan, Jinan 
      250022, PR China.
FAU - Yu, Jinghua
AU  - Yu J
AD  - Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School 
      of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR 
      China. Electronic address: ujn.yujh@gmail.com.
FAU - Yan, Mei
AU  - Yan M
AD  - Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School 
      of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR 
      China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150407
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Enzymes, Immobilized)
RN  - 49DFR088MY (Platinum)
RN  - 7OV03QG267 (Nickel)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.3.5 (bilirubin oxidase)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Bioelectric Energy Sources
MH  - Biosensing Techniques/instrumentation
MH  - Carcinoembryonic Antigen/*blood
MH  - Electrochemical Techniques/instrumentation
MH  - Electrodes
MH  - Enzymes, Immobilized/metabolism
MH  - Equipment Design
MH  - Glucose/metabolism
MH  - Glucose 1-Dehydrogenase/metabolism
MH  - Humans
MH  - Immunoassay/*instrumentation
MH  - *Lab-On-A-Chip Devices
MH  - Limit of Detection
MH  - Models, Molecular
MH  - Nanopores/ultrastructure
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Nickel/chemistry
MH  - Oxidoreductases Acting on CH-CH Group Donors/metabolism
MH  - Oxygen/metabolism
MH  - Paper
MH  - Platinum/chemistry
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Bilirubin oxidase
OT  - Biofuel cell
OT  - Glucose dehydrogenase
OT  - Microfluidic paper-based analytical devices
OT  - Nanoporous PtNi
EDAT- 2015/04/17 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/04/17 06:00
PHST- 2015/01/16 00:00 [received]
PHST- 2015/04/05 00:00 [revised]
PHST- 2015/04/06 00:00 [accepted]
PHST- 2015/04/17 06:00 [entrez]
PHST- 2015/04/17 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
AID - S0956-5663(15)30020-8 [pii]
AID - 10.1016/j.bios.2015.04.019 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2015 Sep 15;71:18-24. doi: 10.1016/j.bios.2015.04.019. Epub 
      2015 Apr 7.