PMID- 25881786
OWN - NLM
STAT- MEDLINE
DCOM- 20150804
LR  - 20201209
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 196
IP  - 2
DP  - 2015 Jun 15
TI  - Development of a standardized trauma-related lung injury model.
PG  - 388-94
LID - S0022-4804(15)00306-6 [pii]
LID - 10.1016/j.jss.2015.03.038 [doi]
AB  - BACKGROUND: The pathophysiology of acute lung injury is multifactorial, and the 
      mechanisms are difficult to prove. We have devised a study of two known and 
      standardized animal models (hemorrhagic shock [HS] and oleic acid [OA]) to more 
      closely reproduce the pathophysiology of posttraumatic acute lung injury. 
      MATERIAL AND METHODS: Pressure-controlled HS (group HS) was performed by 
      withdrawing blood over 15-min until mean arterial pressure reached 35 mm Hg for 
      90 min. In an additional group, HS and standardized lung injury induced by OA 
      were combined (group lung injury [HS + OA]). After the shock period, both groups 
      were resuscitated over 15 min by transfusion of the removed blood and an equal 
      volume of lactate Ringer solution. The end point was 6 h. Plasma interleukin 
      (IL)-6, keratinocyte chemoattractant (KC), IL-10, monocyte chemoattractant 
      protein-1 (MCP-1), and lung histology were carried out. RESULTS: The 
      posttraumatic lung injury group demonstrated significantly higher IL-6 levels 
      when compared with HS group (744.8 +/- 104 versus 297.7 +/- 134 pg/mL; P = 0.004). 
      Histologic analysis confirmed diffuse alveolar congestion and moderate-to-severe 
      lung edema in animals with HS + OA. Lung injury was mild in mice with isolated HS 
      or OA injection. CONCLUSIONS: We established a posttraumatic lung injury model 
      combining two different standardized protocols (HS and OA). This model leads to 
      pronounced inflammation and lung injury. This model allows the analysis of the 
      dynamics of sterile lung injury and associated organ dysfunction.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Pfeifer, Roman
AU  - Pfeifer R
AD  - Department of Orthopaedics and Trauma Surgery and Harald Tscherne Laboratory, 
      Aachen University Medical Center, RWTH Aachen University, Aachen, Germany. 
      Electronic address: rpfeifer@ukaachen.de.
FAU - Andruszkow, Julia H K
AU  - Andruszkow JH
AD  - Institute of Pathology, Aachen University Medical Center, RWTH Aachen University, 
      Aachen, Germany.
FAU - Busch, Daniel
AU  - Busch D
AD  - Department of Surgery, University Medical Center, RWTH Aachen University, Aachen, 
      Germany.
FAU - Hoepken, Merle
AU  - Hoepken M
AD  - Department of Orthopaedics and Trauma Surgery and Harald Tscherne Laboratory, 
      Aachen University Medical Center, RWTH Aachen University, Aachen, Germany.
FAU - Barkatali, Bilal M
AU  - Barkatali BM
AD  - Department of Trauma and Orthopaedics, Salford Royal Teaching Hospitals 
      Foundation NHS Trust, Salford, United Kingdom.
FAU - Horst, Klemens
AU  - Horst K
AD  - Department of Orthopaedics and Trauma Surgery and Harald Tscherne Laboratory, 
      Aachen University Medical Center, RWTH Aachen University, Aachen, Germany.
FAU - Pape, Hans-Christoph
AU  - Pape HC
AD  - Department of Orthopaedics and Trauma Surgery and Harald Tscherne Laboratory, 
      Aachen University Medical Center, RWTH Aachen University, Aachen, Germany.
FAU - Hildebrand, Frank
AU  - Hildebrand F
AD  - Department of Orthopaedics and Trauma Surgery and Harald Tscherne Laboratory, 
      Aachen University Medical Center, RWTH Aachen University, Aachen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150320
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Cytokines)
RN  - 0 (Oleic Acids)
SB  - IM
MH  - Acute Lung Injury/blood/*etiology
MH  - Animals
MH  - Cytokines/blood
MH  - *Disease Models, Animal
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Oleic Acids
MH  - Respiratory Distress Syndrome/blood/*etiology
MH  - Shock, Hemorrhagic/blood/*complications
OTO - NOTNLM
OT  - ARDS
OT  - Hemorrhagic shock
OT  - Inflammation
OT  - Oleic acid
EDAT- 2015/04/18 06:00
MHDA- 2015/08/05 06:00
CRDT- 2015/04/18 06:00
PHST- 2015/02/16 00:00 [received]
PHST- 2015/02/16 00:00 [revised]
PHST- 2015/03/16 00:00 [accepted]
PHST- 2015/04/18 06:00 [entrez]
PHST- 2015/04/18 06:00 [pubmed]
PHST- 2015/08/05 06:00 [medline]
AID - S0022-4804(15)00306-6 [pii]
AID - 10.1016/j.jss.2015.03.038 [doi]
PST - ppublish
SO  - J Surg Res. 2015 Jun 15;196(2):388-94. doi: 10.1016/j.jss.2015.03.038. Epub 2015 
      Mar 20.