PMID- 25884474
OWN - NLM
STAT- MEDLINE
DCOM- 20160129
LR  - 20181202
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 6
IP  - 1
DP  - 2015 Apr 16
TI  - Altered protein secretions during interactions between adipose tissue- or bone 
      marrow-derived stromal cells and inflammatory cells.
PG  - 70
LID - 10.1186/s13287-015-0052-y [doi]
LID - 70
AB  - INTRODUCTION: Paracrine effects can be exploited in cell-based therapies that 
      secrete factors, such as chemokines and cytokines, and can recruit inflammatory 
      cells to transplants. In this study, mouse adipose tissue-derived stromal cells 
      (ASCs) and bone marrow-derived stromal cells (ST2 cells) were used to examine 
      changes in paracrine interactions with inflammation cells. METHODS: Green 
      fluorescent protein positive (GFP+) bone marrow cells (BMCs) were injected into 
      an irradiated mouse via the femoral vein, and ASCs and ST2 cells were 
      transplanted intradermally. Subsequently, an in vivo imaging system was used to 
      observe behaviors of GFP+ BMCs. To detect bone marrow-derived inflammatory cells 
      which migrated to the ASC and ST2 cell transplantation area, the sections were 
      immunostained using antibodies against Gr1, CD11c, and F4/80, and secretory 
      proteins were detected in culture medium using enzyme-linked immunosorbent assay. 
      RESULTS: Many bone marrow-derived inflammatory cells migrated to ASC and ST2 cell 
      transplantation sites. Among these, neutrophils were detected during the early 
      period and macrophages were predominantly detected at a later point in time. Many 
      chemokines, cytokines, growth factors, matrix metalloproteinases (MMPs), and 
      tissue inhibitors of metalloproteinases (TIMPs) were secreted in abundance from 
      ASCs, and the secretion increased by co-culturing with inflammatory cells, except 
      for secretions of insulin-like growth factor-1, MMP-9 and MMP-13. Although 
      secretions from ST2 cells were less than those from ASCs, co-culture with 
      inflammatory cells increased these secretions to levels similar to those of ASCs. 
      However, unlike ASCs, the ST2 cells did not secrete angiostatin, MMP-2, or MMP-3. 
      Finally, ASCs secreted not only proinflammatory cytokines, angiogenic factors and 
      MMPs but also anti-inflammatory cytokines, anti-angiogenesis factors, and TIMPs. 
      CONCLUSIONS: The effects of cell-based therapies using ASCs and ST2 cells are 
      depended on paracrine effects that are mediated by chemokines, cytokines, growth 
      factors, MMPs, and TIMPs, which comprise responses to interactions between 
      transplanted cells and inflammatory cells. Moreover, paracrine effects of 
      transplanted cells are influenced by inflammatory cells, and are moderated by a 
      balance of secreted inhibitors.
FAU - Hattori, Hidemi
AU  - Hattori H
AD  - Division of Biomedical Engineering, Research Institute, National Defense Medical 
      College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan. h2@ndmc.ac.jp.
FAU - Ishihara, Masayuki
AU  - Ishihara M
AD  - Division of Biomedical Engineering, Research Institute, National Defense Medical 
      College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan. ishihara@ndmc.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20150416
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Inflammation/*immunology
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Macrophages/immunology
MH  - Male
MH  - Matrix Metalloproteinases/metabolism
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neovascularization, Physiologic/physiology
MH  - Neutrophils/immunology
MH  - Paracrine Communication/*physiology
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Wound Healing/physiology
PMC - PMC4417284
EDAT- 2015/04/18 06:00
MHDA- 2016/01/30 06:00
PMCR- 2015/04/16
CRDT- 2015/04/18 06:00
PHST- 2014/06/03 00:00 [received]
PHST- 2015/03/13 00:00 [accepted]
PHST- 2015/01/04 00:00 [revised]
PHST- 2015/04/18 06:00 [entrez]
PHST- 2015/04/18 06:00 [pubmed]
PHST- 2016/01/30 06:00 [medline]
PHST- 2015/04/16 00:00 [pmc-release]
AID - 10.1186/s13287-015-0052-y [pii]
AID - 52 [pii]
AID - 10.1186/s13287-015-0052-y [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2015 Apr 16;6(1):70. doi: 10.1186/s13287-015-0052-y.