PMID- 25886766
OWN - NLM
STAT- MEDLINE
DCOM- 20160112
LR  - 20181113
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 16
DP  - 2015 Feb 18
TI  - The effect of adolescent testosterone on hippocampal BDNF and TrkB mRNA 
      expression: relationship with cell proliferation.
PG  - 4
LID - 10.1186/s12868-015-0142-x [doi]
LID - 4
AB  - BACKGROUND: Testosterone attenuates postnatal hippocampal neurogenesis in 
      adolescent male rhesus macaques through altering neuronal survival. While 
      brain-derived neurotropic factor (BDNF)/ tyrosine kinase receptor B (TrkB) are 
      critical in regulating neuronal survival, it is not known if the molecular 
      mechanism underlying testosterone's action on postnatal neurogenesis involves 
      changes in BDNF/TrkB levels. First, (1) we sought to localize the site of 
      synthesis of the full length and truncated TrkB receptor in the neurogenic 
      regions of the adolescent rhesus macaque hippocampus. Next, (2) we asked if 
      gonadectomy or sex hormone replacement altered hippocampal BDNF and TrkB 
      expression level in mammalian hippocampus (rhesus macaque and Sprague Dawley 
      rat), and (3) if the relationship between BDNF/TrkB expression was altered 
      depending on the sex steroid environment. RESULTS: We find that truncated TrkB 
      mRNA+ cells are highly abundant in the proliferative subgranular zone (SGZ) of 
      the primate hippocampus; in addition, there are scant and scattered full length 
      TrkB mRNA+ cells in this region. Gonadectomy or sex steroid replacement did not 
      alter BDNF or TrkB mRNA levels in young adult male rat or rhesus macaque 
      hippocampus. In the monkey and rat, we find a positive correlation with cell 
      proliferation and TrkB-TK+ mRNA expression, and this positive relationship was 
      found only when sex steroids were present. CONCLUSIONS: We suggest that 
      testosterone does not down-regulate neurogenesis at adolescence via overall 
      changes in BDNF or TrkB expression. However, BDNF/TrkB mRNA appears to have a 
      greater link to cell proliferation in the presence of circulating testosterone.
FAU - Allen, Katherine M
AU  - Allen KM
AD  - Schizophrenia Research Institute, Sydney, Australia. k.allen@neura.edu.au.
AD  - Schizophrenia Research Laboratory, Neuroscience Research Australia, Barker 
      Street, Randwick, NSW, 2031, Australia. k.allen@neura.edu.au.
AD  - School of Psychiatry, University of New South Wales, Sydney, Australia. 
      k.allen@neura.edu.au.
FAU - Purves-Tyson, Tertia D
AU  - Purves-Tyson TD
AD  - Schizophrenia Research Institute, Sydney, Australia. t.purves-tyson@neura.edu.au.
AD  - Schizophrenia Research Laboratory, Neuroscience Research Australia, Barker 
      Street, Randwick, NSW, 2031, Australia. t.purves-tyson@neura.edu.au.
AD  - School of Medical Sciences, University of New South Wales, Sydney, Australia. 
      t.purves-tyson@neura.edu.au.
FAU - Fung, Samantha J
AU  - Fung SJ
AD  - Schizophrenia Research Institute, Sydney, Australia. s.fung@neura.edu.au.
AD  - Schizophrenia Research Laboratory, Neuroscience Research Australia, Barker 
      Street, Randwick, NSW, 2031, Australia. s.fung@neura.edu.au.
AD  - School of Psychiatry, University of New South Wales, Sydney, Australia. 
      s.fung@neura.edu.au.
FAU - Shannon Weickert, Cynthia
AU  - Shannon Weickert C
AD  - Schizophrenia Research Institute, Sydney, Australia. cyndi@neura.edu.au.
AD  - Schizophrenia Research Laboratory, Neuroscience Research Australia, Barker 
      Street, Randwick, NSW, 2031, Australia. cyndi@neura.edu.au.
AD  - School of Psychiatry, University of New South Wales, Sydney, Australia. 
      cyndi@neura.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150218
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (RNA, Messenger)
RN  - 3XMK78S47O (Testosterone)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Bromodeoxyuridine
MH  - Hippocampus/drug effects/*growth & development/*metabolism
MH  - Hormone Replacement Therapy
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Ki-67 Antigen/metabolism
MH  - Macaca mulatta
MH  - Male
MH  - Neurogenesis/drug effects/physiology
MH  - Orchiectomy
MH  - RNA, Messenger/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, trkB/*metabolism
MH  - Species Specificity
MH  - Stem Cell Niche/drug effects/physiology
MH  - Testosterone/administration & dosage/*metabolism
PMC - PMC4367905
EDAT- 2015/04/19 06:00
MHDA- 2016/01/13 06:00
PMCR- 2015/02/18
CRDT- 2015/04/19 06:00
PHST- 2014/08/28 00:00 [received]
PHST- 2015/02/05 00:00 [accepted]
PHST- 2015/04/19 06:00 [entrez]
PHST- 2015/04/19 06:00 [pubmed]
PHST- 2016/01/13 06:00 [medline]
PHST- 2015/02/18 00:00 [pmc-release]
AID - 10.1186/s12868-015-0142-x [pii]
AID - 142 [pii]
AID - 10.1186/s12868-015-0142-x [doi]
PST - epublish
SO  - BMC Neurosci. 2015 Feb 18;16:4. doi: 10.1186/s12868-015-0142-x.