PMID- 25888850
OWN - NLM
STAT- MEDLINE
DCOM- 20160407
LR  - 20181113
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 13
DP  - 2015 Mar 3
TI  - Exposure to di(2-ethylhexyl) phthalate inhibits luteal function via dysregulation 
      of CD31 and prostaglandin F2alpha in pregnant mice.
PG  - 11
LID - 10.1186/s12958-015-0013-4 [doi]
LID - 11
AB  - BACKGROUND: Di(2-ethylhexyl) phthalate (DEHP) exposure reduces embryo 
      implantations, increases embryonic loss, and decreases fetal body weights. 
      However, whether it is associated with the alteration of luteal function remains 
      unknown. Thus, our aim in this study was to explore the effect and mechanism of 
      DEHP on luteal function in pregnant mice in vivo. METHODS: Mice were administered 
      DEHP by gavage at 125, 250, 500 mg/kg/day from gestational days (GD) 1 to 9 or 
      13. Levels of serum progesterone and estradiol were measured by radioimmunoassay. 
      The numbers and sizes of corpora lutea were calculated by ovarian 
      histomorphology. Steroidogenic enzymes were assessed by qRT-PCR. CD31 protein was 
      detected by immunocytochemistry, and prostaglandin F2alpha (PGF2alpha) levels 
      were evaluated by enzyme immunoassay. RESULTS: Treatment with DEHP significantly 
      inhibited progesterone secretion in pregnant mice in a dose-dependent manner but 
      did not inhibit estradiol production on GD 9 and 13. Treatment also showed 
      concomitant decreases in transcript levels for key steroidogenic enzymes (CYP11A, 
      3beta-HSD, and StAR) on GD 13. Furthermore, DEHP administration significantly 
      reduced the numbers and sizes of corpora lutea on GD 13. No significant changes 
      in the ratio of ovary weight vs. body weight were observed between the control 
      group and treated animals on GD 9 and 13. In addition, treatment with DEHP 
      significantly inhibited CD31 expression of corpora lutea, whereas plasma 
      PGF2alpha levels in DEHP treatment groups were significantly higher compared with 
      the control groups on GD 9 and 13. CONCLUSIONS: The results show DEHP 
      significantly inhibits luteal function of pregnant mice in vivo, with a mechanism 
      that seems to involve the down-regulation of progesterone and steroidogenic 
      enzymes message RNA, the decrease in CD31 expression, and the increase in 
      PGF2alpha secretion.
FAU - Guo, Meijun
AU  - Guo M
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. 741046571@qq.com.
FAU - Lai, Lidan
AU  - Lai L
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. 1037715496@qq.com.
FAU - Zong, Teng
AU  - Zong T
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. 258056615@qq.com.
FAU - Lin, Yan
AU  - Lin Y
AD  - Department of Obstetrics and Gynecology, Hospital of Jixi Province People, 
      Nanchang, Jiangxi, China. lingyan1205@163.com.
FAU - Yang, Bei
AU  - Yang B
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. yangbei@ncu.edu.cn.
FAU - Zhang, Lu
AU  - Zhang L
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. 13393733537@163.com.
FAU - Li, Mo
AU  - Li M
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. 544182657@qq.com.
FAU - Kuang, Haibin
AU  - Kuang H
AD  - Department of Physiology, School of Medicine, Nanchang University, Nanchang, 
      Jiangxi, China. kuanghaibin@ncu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150303
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - B7IN85G1HY (Dinoprost)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
SB  - IM
MH  - Animals
MH  - Corpus Luteum/anatomy & histology/drug effects
MH  - Corpus Luteum Maintenance/drug effects
MH  - Diethylhexyl Phthalate/*toxicity
MH  - Dinoprost/*metabolism
MH  - Estradiol/blood
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - Mice
MH  - Neovascularization, Physiologic/drug effects
MH  - Organ Size
MH  - Ovary/anatomy & histology/drug effects/metabolism
MH  - Platelet Endothelial Cell Adhesion Molecule-1/*metabolism
MH  - Pregnancy
MH  - Progesterone/blood
PMC - PMC4351920
EDAT- 2015/04/19 06:00
MHDA- 2016/04/08 06:00
PMCR- 2015/03/03
CRDT- 2015/04/19 06:00
PHST- 2014/11/05 00:00 [received]
PHST- 2015/02/23 00:00 [accepted]
PHST- 2015/04/19 06:00 [entrez]
PHST- 2015/04/19 06:00 [pubmed]
PHST- 2016/04/08 06:00 [medline]
PHST- 2015/03/03 00:00 [pmc-release]
AID - 10.1186/s12958-015-0013-4 [pii]
AID - 13 [pii]
AID - 10.1186/s12958-015-0013-4 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2015 Mar 3;13:11. doi: 10.1186/s12958-015-0013-4.