PMID- 25889225 OWN - NLM STAT- MEDLINE DCOM- 20160217 LR - 20200319 IS - 1873-474X (Electronic) IS - 0736-5748 (Linking) VI - 43 DP - 2015 Jun TI - Antenatal betamethasone produces protracted changes in anxiety-like behaviors and in the expression of microtubule-associated protein 2, brain-derived neurotrophic factor and the tyrosine kinase B receptor in the rat cerebellar cortex. PG - 78-85 LID - S0736-5748(15)00053-2 [pii] LID - 10.1016/j.ijdevneu.2015.04.005 [doi] AB - Using classic Golgi staining methods, we previously showed that the administration of synthetic glucocorticoid betamethasone in equivalent doses to those given in cases of human premature birth generates long-term alterations in Purkinje cell dendritic development in the cerebellar cortex. In the present study, we evaluated whether betamethasone alters the immunohistochemical expression of proteins that participate in cerebellar Purkinje cell dendritic development and maintenance, including microtubule-associated protein 2 (MAP2), brain-derived neurotrophic factor (BDNF) and the tyrosine kinase B receptor (TrkB), which are located predominantly in the cerebellar molecular layer where Purkinje cell dendritogenesis occurs. Consistent with our previous Golgi stain studies, we observed that animals prenatally exposed to a single course of betamethasone showed long-term alterations in the expression of MAP2, BDNF and TrkB. Additionally, these protracted molecular changes were accompanied by anxiety-like behaviors in the elevated plus maze and marble burying tests. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Pascual, Rodrigo AU - Pascual R AD - Laboratorio de Neurociencias, Escuela de Kinesiologia, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile. Electronic address: rodrigo.pascual@ucv.cl. FAU - Valencia, Martina AU - Valencia M AD - Laboratorio de Neurociencias, Escuela de Kinesiologia, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile. Electronic address: valencia.narbona.lab@gmail.com. FAU - Bustamante, Carlos AU - Bustamante C AD - Laboratorio de Neurociencias, Escuela de Kinesiologia, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile. Electronic address: carlos.bustamante@ucv.cl. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150415 PL - United States TA - Int J Dev Neurosci JT - International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience JID - 8401784 RN - 0 (Anti-Anxiety Agents) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (MAP2 protein, rat) RN - 0 (Microtubule-Associated Proteins) RN - 9842X06Q6M (Betamethasone) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Age Factors MH - Analysis of Variance MH - Animals MH - Animals, Newborn MH - Anti-Anxiety Agents/*pharmacology MH - Anxiety/*drug therapy MH - Betamethasone/*administration & dosage MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cerebellar Cortex/*drug effects/*metabolism MH - Disease Models, Animal MH - Female MH - Male MH - Microtubule-Associated Proteins/*metabolism MH - Pregnancy MH - Rats MH - Receptor, trkB/*metabolism OTO - NOTNLM OT - Anxiety-like behavior OT - BDNF OT - Betamethasone OT - Cerebellar cortex OT - MAP2 OT - TrkB EDAT- 2015/04/19 06:00 MHDA- 2016/02/18 06:00 CRDT- 2015/04/19 06:00 PHST- 2015/03/19 00:00 [received] PHST- 2015/04/10 00:00 [revised] PHST- 2015/04/12 00:00 [accepted] PHST- 2015/04/19 06:00 [entrez] PHST- 2015/04/19 06:00 [pubmed] PHST- 2016/02/18 06:00 [medline] AID - S0736-5748(15)00053-2 [pii] AID - 10.1016/j.ijdevneu.2015.04.005 [doi] PST - ppublish SO - Int J Dev Neurosci. 2015 Jun;43:78-85. doi: 10.1016/j.ijdevneu.2015.04.005. Epub 2015 Apr 15.