PMID- 25890007
OWN - NLM
STAT- MEDLINE
DCOM- 20160223
LR  - 20150606
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 76
IP  - 6
DP  - 2015 Jun
TI  - A reliable, high-resolution and high-throughput genotyping method for HLA-DRB1.
PG  - 397-401
LID - S0198-8859(15)00134-2 [pii]
LID - 10.1016/j.humimm.2015.04.003 [doi]
AB  - Human leukocyte antigen (HLA) DNA typing is an essential part to identify a donor 
      who may be a good match for the patients who need haematopoietic stem cells from 
      bone marrow. Thus, fetching quickly high-resolution genotype is very important at 
      present. However, current HLA DNA typing methods especially for HLA-DRB1 
      generally yielded ambiguous typing results because of high degree of 
      heterozygosity on exome region and primer pairs design limitations, which 
      generally amplified only exon2 of HLA-DRB1 and the position of its primer pairs 
      is on exome region. To solve this problem, we developed a reliable, 
      high-resolution and high-throughput (RHH) sequence based typing approach for 
      HLA-DRB1 through massively parallel paired-end sequencing. Several primer pairs 
      were designed to amplify three exon regions, which are part of exon1, the whole 
      region of exon2 and exon3 of HLA-DRB1, to genotyping for HLA-DRB1 by synthesis. 
      94 samples were included to analyze and the highly successful genotyping ratio 
      (98.94%) and no ambiguous genotyping result demonstrated the accurate performance 
      of our method for HLA-DRB1 genotyping.
CI  - Copyright (c) 2015 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Yang, Fan
AU  - Yang F
AD  - BGI Health Service Co., Ltd, China.
FAU - Jiang, Fuman
AU  - Jiang F
AD  - BGI Health Service Co., Ltd, China.
FAU - Chai, Xianghua
AU  - Chai X
AD  - BGI Health Service Co., Ltd, China.
FAU - Guo, Yulai
AU  - Guo Y
AD  - BGI Health Service Co., Ltd, China.
FAU - Lan, Zhiheng
AU  - Lan Z
AD  - BGI Health Service Co., Ltd, China.
FAU - Yuan, Yuying
AU  - Yuan Y
AD  - BGI Health Service Co., Ltd, China.
FAU - Huang, Changjian
AU  - Huang C
AD  - BGI Health Service Co., Ltd, China.
FAU - Zhu, Zhongyi
AU  - Zhu Z
AD  - BGI Health Service Co., Ltd, China.
FAU - Li, Huiling
AU  - Li H
AD  - BGI Health Service Co., Ltd, China.
FAU - Du, Bole
AU  - Du B
AD  - BGI Health Service Co., Ltd, China.
FAU - Wang, Jun
AU  - Wang J
AD  - BGI Health Service Co., Ltd, China.
FAU - Zhou, Lijun
AU  - Zhou L
AD  - BGI Health Service Co., Ltd, China.
FAU - Chen, Zhonglin
AU  - Chen Z
AD  - BGI Health Service Co., Ltd, China.
FAU - Yang, Ling
AU  - Yang L
AD  - BGI Health Service Co., Ltd, China.
FAU - Zhou, Meizhen
AU  - Zhou M
AD  - BGI Health Service Co., Ltd, China.
FAU - Zeng, Hao
AU  - Zeng H
AD  - BGI Health Service Co., Ltd, China.
FAU - Xu, Yicou
AU  - Xu Y
AD  - BGI Health Service Co., Ltd, China.
FAU - Wang, Wei
AU  - Wang W
AD  - BGI Health Service Co., Ltd, China. Electronic address: wangw@genomics.cn.
LA  - eng
PT  - Journal Article
DEP - 20150415
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Alleles
MH  - DNA Primers
MH  - *Exons
MH  - Gene Frequency
MH  - Genetic Loci
MH  - Genotype
MH  - *Genotyping Techniques
MH  - HLA-DRB1 Chains/classification/*genetics/immunology
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Histocompatibility Testing
MH  - Humans
MH  - Tissue Donors
OTO - NOTNLM
OT  - Ambiguous
OT  - Genotyping
OT  - High-resolution
OT  - Human leukocyte antigen
OT  - Next-generation sequencing
EDAT- 2015/04/19 06:00
MHDA- 2016/02/26 06:00
CRDT- 2015/04/19 06:00
PHST- 2014/06/12 00:00 [received]
PHST- 2015/02/05 00:00 [revised]
PHST- 2015/04/09 00:00 [accepted]
PHST- 2015/04/19 06:00 [entrez]
PHST- 2015/04/19 06:00 [pubmed]
PHST- 2016/02/26 06:00 [medline]
AID - S0198-8859(15)00134-2 [pii]
AID - 10.1016/j.humimm.2015.04.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2015 Jun;76(6):397-401. doi: 10.1016/j.humimm.2015.04.003. Epub 2015 
      Apr 15.