PMID- 25891217
OWN - NLM
STAT- MEDLINE
DCOM- 20170710
LR  - 20181113
IS  - 2042-0226 (Electronic)
IS  - 1672-7681 (Print)
IS  - 1672-7681 (Linking)
VI  - 13
IP  - 4
DP  - 2016 Jul
TI  - Delineation of a novel dendritic-like subset in human spleen.
PG  - 443-50
LID - 10.1038/cmi.2015.16 [doi]
AB  - Dendritic cells (DCs) and monocyte subpopulations present in the human spleen 
      were analyzed by flow cytometry in an attempt to identify the presence of a novel 
      dendritic-like cell subset described previously in mice and named L-DCs. In this 
      study, an equivalent of this novel murine subset was characterized in the human 
      spleen, thus increasing our knowledge of the antigen-presenting cell types 
      present in the human spleen. Human L-DCs were identified as a 
      hCD11c(+)hCD11b(+)HLA-DR(-)hCD86(+) subset in the spleen, along with the 
      previously described subsets of hCD1c(+) DCs, hCD123(+) plasmacytoid DCs (pDCs), 
      hCD16(+) DCs and hCD141(+) DCs. Three subsets of monocytes were also 
      characterized. DC and monocyte subsets in human spleen had phenotypes similar to 
      those of subsets in human blood. In line with murine studies, the presence of 
      L-DC progenitors within the spleen was also investigated. When human splenocytes 
      depleted of T and B cells were cocultured with the murine stromal line 5G3, 
      hematopoiesis ensued and hCD11c(+)HLA-DR(+) and hCD11c(+)HLA-DR(-) cells were 
      produced. The latter resemble L-DCs, which are also produced in murine spleen 
      cocultures. Both subsets expressed hCD80 and hCD86, which identifies them as 
      antigen-presenting cells, particularly DCs, and were highly endocytic. It is 
      noteworthy that murine splenic stroma can serve as a support matrix for human 
      hematopoiesis and DC production. These results support the hypothesis that 5G3 
      must express both cell-associated and soluble factors that can signal 
      hematopoiesis in human and murine progenitors.
FAU - Petvises, Sawang
AU  - Petvises S
AD  - Research School of Biology, The Australian National University, Canberra, 
      Australia.
FAU - Talaulikar, Dipti
AU  - Talaulikar D
AD  - John Curtin School of Medical Research, The Australian National University, 
      Canberra, Australia.
AD  - ANU Medical School, College of Medicine Biology and Environment, The Australian 
      National University, Canberra, Australia.
AD  - Department of Haematology, Canberra Hospital, Canberra, Australia.
FAU - O'Neill, Helen C
AU  - O'Neill HC
AD  - Research School of Biology, The Australian National University, Canberra, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150420
PL  - China
TA  - Cell Mol Immunol
JT  - Cellular & molecular immunology
JID - 101242872
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Coculture Techniques
MH  - Dendritic Cells/*cytology/*immunology
MH  - Endocytosis
MH  - Humans
MH  - Mice
MH  - Monocytes/cytology
MH  - Spleen/*cytology/immunology
MH  - Stromal Cells/cytology
PMC - PMC4947811
EDAT- 2015/04/22 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/07/01
CRDT- 2015/04/21 06:00
PHST- 2014/12/18 00:00 [received]
PHST- 2015/02/01 00:00 [revised]
PHST- 2015/02/01 00:00 [accepted]
PHST- 2015/04/21 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - cmi201516 [pii]
AID - 10.1038/cmi.2015.16 [doi]
PST - ppublish
SO  - Cell Mol Immunol. 2016 Jul;13(4):443-50. doi: 10.1038/cmi.2015.16. Epub 2015 Apr 
      20.