PMID- 25891482
OWN - NLM
STAT- MEDLINE
DCOM- 20160125
LR  - 20150504
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 35
IP  - 6
DP  - 2015 Jun
TI  - Influence of particle size on the in vitro and in vivo anti-inflammatory and 
      anti-allergic activities of a curcumin lipid nanoemulsion.
PG  - 1720-8
LID - 10.3892/ijmm.2015.2186 [doi]
AB  - The polyphenolic compound, curcumin, is a natural yellow pigment component of 
      turmeric. It exerts various biological effects, such as anti-inflammatory 
      effects, and we have previously demonstrated that curcumin is a specific 
      inhibitor of DNA polymerase lambda. Curcumin is characterized by poor bioavailability 
      as it is water-insoluble, is poorly absorbed and is systemically eliminated. In 
      order to increase the bioavailability of curcumin, in this study, we produced a 
      curcumin-loaded lipid nanoemulsion of various particle sizes (50, 100 and 200 
      nm). The curcumin lipid nanoemulsion was prepared by a modified thin-film 
      hydration method followed by sonication. To identify the optimal particle size 
      which exhibits the strongest physiological activity, we investigated the 
      inhibitory effects of the obtained nanoemulsions against inflammatory and 
      allergic activities. In in vitro cell culture experiments, the 100-nm curcumin 
      lipid nanoemulsion showed the most prominent inhibitory effect on the production 
      of tumor necrosis factor-alpha (TNF-alpha) induced by lipopolysaccharide (LPS) in 
      RAW264.7 murine macrophages, and on the release of beta-hexosaminidase induced by 
      the calcium ionophore, A23187, in rat basophilic leukemia RBL-2H3 cells. In an in 
      vivo experiment, in which mice were administered the curcumin-loaded lipid 
      nanoemulsion of various particle sizes, the 100-nm curcumin lipid nanoemulsion 
      showed the most prominent anti-inflammatory and anti-allergic effects, inhibiting 
      12-O-tetradecanoylphorbol-13-acetate-induced inflammatory ear edema and 
      immunoglobulin E (IgE)-induced passive cutaneous anaphylactic (PCA) reaction. The 
      effects of particle size on serum curcumin absorption were also assessed in mice, 
      and the 100-nm lipid nanoemulsion showed the greatest absorption. The results 
      from our study suggest that the physiological activities of curcumin lipid 
      nanoemulsions differ depending on particle size. Our data indicate that the 
      curcumin lipid nanoemulsion with a particle size of 100 nm has potential for use 
      in enhancing the bioavailability and medical value of curcumin.
FAU - Onodera, Takefumi
AU  - Onodera T
AD  - Laboratory of Food and Nutritional Sciences, Faculty of Nutrition, Kobe Gakuin 
      University, Kobe, Hyogo 651-2180, Japan.
FAU - Kuriyama, Isoko
AU  - Kuriyama I
AD  - Cooperative Research Center of Life Sciences, Kobe Gakuin University, Kobe, Hyogo 
      651-8586, Japan.
FAU - Andoh, Tooru
AU  - Andoh T
AD  - Cooperative Research Center of Life Sciences, Kobe Gakuin University, Kobe, Hyogo 
      651-8586, Japan.
FAU - Ichikawa, Hideki
AU  - Ichikawa H
AD  - Cooperative Research Center of Life Sciences, Kobe Gakuin University, Kobe, Hyogo 
      651-8586, Japan.
FAU - Sakamoto, Yuka
AU  - Sakamoto Y
AD  - Laboratory of Food and Nutritional Sciences, Faculty of Nutrition, Kobe Gakuin 
      University, Kobe, Hyogo 651-2180, Japan.
FAU - Lee-Hiraiwa, Eibai
AU  - Lee-Hiraiwa E
AD  - Cooperative Research Center of Life Sciences, Kobe Gakuin University, Kobe, Hyogo 
      651-8586, Japan.
FAU - Mizushina, Yoshiyuki
AU  - Mizushina Y
AD  - Cooperative Research Center of Life Sciences, Kobe Gakuin University, Kobe, Hyogo 
      651-8586, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150416
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Drug Carriers)
RN  - 0 (Emulsions)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - IT942ZTH98 (Curcumin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - *Anti-Inflammatory Agents/chemistry/pharmacology
MH  - Cell Line, Tumor
MH  - *Curcumin/chemistry/pharmacology
MH  - *Drug Carriers/chemistry/pharmacology
MH  - Edema/chemically induced/*drug therapy/metabolism/pathology
MH  - Emulsions
MH  - Macrophages/metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Nanoparticles/*chemistry
MH  - Particle Size
MH  - Rats
MH  - Tetradecanoylphorbol Acetate/toxicity
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2015/04/22 06:00
MHDA- 2016/01/26 06:00
CRDT- 2015/04/21 06:00
PHST- 2015/02/05 00:00 [received]
PHST- 2015/04/09 00:00 [accepted]
PHST- 2015/04/21 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/01/26 06:00 [medline]
AID - 10.3892/ijmm.2015.2186 [doi]
PST - ppublish
SO  - Int J Mol Med. 2015 Jun;35(6):1720-8. doi: 10.3892/ijmm.2015.2186. Epub 2015 Apr 
      16.