PMID- 25891649
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20181113
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Print)
IS  - 0897-7151 (Linking)
VI  - 32
IP  - 19
DP  - 2015 Oct 1
TI  - Chaperone-Mediated Autophagy after Traumatic Brain Injury.
PG  - 1449-57
LID - 10.1089/neu.2014.3694 [doi]
AB  - Chaperone-mediated autophagy (CMA) and the ubiquitin-proteasomal system (UPS) are 
      two major protein degradation systems responsible for maintaining cellular 
      homeostasis, but how these two systems are regulated after traumatic brain injury 
      (TBI) remains unknown. TBI produces primary mechanical damage that must be 
      repaired to maintain neuronal homeostasis. The level of lysosomal-associated 
      membrane protein type 2A (LAMP2A) is the hallmark of CMA activity. The level of 
      polyubiquitinated proteins (ubi-proteins) reflects UPS activity. This study 
      utilized a moderate fluid percussion injury model in rats to investigate the 
      changes in CMA and the UPS after TBI. Induction of CMA was manifested by 
      significant upregulation of LAMP2A and secondary lysosomes during the periods of 
      1-15 days of recovery after TBI. In comparison, the levels of ubi-proteins were 
      increased only moderately after TBI. The increases in the levels of LAMP2A and 
      70 kDa heat-shock protein for CMA after TBI were seen mainly in the secondary 
      lysosome-containing fractions. Confocal and electron microscopy further showed 
      that increased LAMP2A or lysosomes were found mainly in neurons and proliferated 
      microglia. Because CMA and the UPS are two major routes for elimination of 
      different types of cellular aberrant proteins, the consecutive activation of 
      these two pathways may serve as a protective mechanism for maintaining cellular 
      homeostasis after TBI.
FAU - Park, Yujung
AU  - Park Y
AD  - 1 Neurochemistry Laboratory of Brain Injury, Shock Trauma and Anesthesiology 
      Research Center; University of Maryland School of Medicine , Baltimore, Maryland.
FAU - Liu, Chunli
AU  - Liu C
AD  - 1 Neurochemistry Laboratory of Brain Injury, Shock Trauma and Anesthesiology 
      Research Center; University of Maryland School of Medicine , Baltimore, Maryland.
FAU - Luo, Tianfei
AU  - Luo T
AD  - 1 Neurochemistry Laboratory of Brain Injury, Shock Trauma and Anesthesiology 
      Research Center; University of Maryland School of Medicine , Baltimore, Maryland.
FAU - Dietrich, W Dalton
AU  - Dietrich WD
AD  - 2 Department of Neurological Surgery, The Miami Project to Cure Paralysis, 
      University of Miami School of Medicine , Miami, Florida.
FAU - Bramlett, Helen
AU  - Bramlett H
AD  - 2 Department of Neurological Surgery, The Miami Project to Cure Paralysis, 
      University of Miami School of Medicine , Miami, Florida.
FAU - Hu, Bingren
AU  - Hu B
AD  - 1 Neurochemistry Laboratory of Brain Injury, Shock Trauma and Anesthesiology 
      Research Center; University of Maryland School of Medicine , Baltimore, Maryland.
LA  - eng
GR  - R56 NS036810/NS/NINDS NIH HHS/United States
GR  - R01 NS040407/NS/NINDS NIH HHS/United States
GR  - NS030291/NS/NINDS NIH HHS/United States
GR  - R29 NS036810/NS/NINDS NIH HHS/United States
GR  - R01 NS036810/NS/NINDS NIH HHS/United States
GR  - I01 BX001696/BX/BLRD VA/United States
GR  - NS040407/NS/NINDS NIH HHS/United States
GR  - P50 NS030291/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150630
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Lysosomal-Associated Membrane Protein 2)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Ubiquitins)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - *Autophagy
MH  - Brain Injuries/*pathology
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - Homeostasis
MH  - Lysosomal-Associated Membrane Protein 2/metabolism
MH  - Lysosomes/metabolism
MH  - Male
MH  - Microglia/pathology
MH  - *Molecular Chaperones
MH  - Neurons/pathology
MH  - Proteasome Endopeptidase Complex
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Ubiquitins/metabolism
MH  - Up-Regulation
PMC - PMC4589263
OTO - NOTNLM
OT  - HSC70
OT  - HSP70
OT  - Iba-1
OT  - LAMP2
OT  - chaperone-mediated autophagy
OT  - lysosome
OT  - traumatic brain injury
OT  - ubiquitin
EDAT- 2015/04/22 06:00
MHDA- 2016/07/07 06:00
PMCR- 2016/10/01
CRDT- 2015/04/21 06:00
PHST- 2015/04/21 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - 10.1089/neu.2014.3694 [pii]
AID - 10.1089/neu.2014.3694 [doi]
PST - ppublish
SO  - J Neurotrauma. 2015 Oct 1;32(19):1449-57. doi: 10.1089/neu.2014.3694. Epub 2015 
      Jun 30.