PMID- 25893329
OWN - NLM
STAT- MEDLINE
DCOM- 20160831
LR  - 20200930
IS  - 1573-3017 (Electronic)
IS  - 0963-9292 (Linking)
VI  - 24
IP  - 7-8
DP  - 2015 Oct
TI  - Effects of norfloxacin on hepatic genes expression of P450 isoforms (CYP1A and 
      CYP3A), GST and P-glycoprotein (P-gp) in Swordtail fish (Xiphophorus Helleri).
PG  - 1566-73
LID - 10.1007/s10646-015-1457-1 [doi]
AB  - The presence of antibiotics including norfloxacin in the aquatic environment may 
      cause adverse effects in non-target organisms. But the toxic mechanisms of 
      fluoroquinolone to fish species are still not completely elucidated. Thus, it is 
      essential to investigate the response of fish to the exposure of fluoroquinolone 
      at molecular or cellular level for better and earlier prediction of these 
      environmental pollutants toxicity. The sub-chronic toxic effects of norfloxacin 
      (NOR) on swordtail fish (Xiphophoru s helleri) were investigated by measuring 
      mRNA expression of cytochrome P450 1A (CYP1A), cytochrome P450 3A (CYP3A), 
      glutathione S-transferase (GST) and P-glycoprotein (P-gp) and their corresponding 
      enzyme activities (including ethoxyresorufin O-deethylase, erythromycin 
      N-demethylase and GST. Results showed that NOR significantly affected the 
      expression of CYP1A, CYP3A, GST and P-gp genes in swordtails. The gene 
      expressions were more responsive to NOR exposure than their corresponding enzyme 
      activities. Moreover, sexual differences were found in gene expression and enzyme 
      activities of swordtails exposed to NOR. Females displayed more dramatic changes 
      than males. The study further demonstrated that the combined biochemical and 
      molecular parameters were considered as useful biomarkers to improve our 
      understanding of potential ecotoxicological risks of NOR exposure to aquatic 
      organisms.
FAU - Liang, Ximei
AU  - Liang X
AD  - Department of Ecology, Jinan University, Guangzhou, 510632, China.
AD  - Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510380, China.
FAU - Wang, Lan
AU  - Wang L
AD  - Department of Ecology, Jinan University, Guangzhou, 510632, China.
FAU - Ou, Ruikang
AU  - Ou R
AD  - Department of Ecology, Jinan University, Guangzhou, 510632, China.
FAU - Nie, Xiangping
AU  - Nie X
AD  - Department of Ecology, Jinan University, Guangzhou, 510632, China. 
      txpnie@jnu.edu.cn.
AD  - Research Center of Tropical and Subtropical Aquatic Ecological Engineering, 
      Minister of Education, Guangzhou, 510632, China. txpnie@jnu.edu.cn.
FAU - Yang, YuFeng
AU  - Yang Y
AD  - Department of Ecology, Jinan University, Guangzhou, 510632, China.
FAU - Wang, Fang
AU  - Wang F
AD  - Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510380, China.
FAU - Li, Kaibin
AU  - Li K
AD  - Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Guangzhou, 510380, China. likaibins@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150419
PL  - United States
TA  - Ecotoxicology
JT  - Ecotoxicology (London, England)
JID - 9885956
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fish Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - N0F8P22L1P (Norfloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/toxicity
MH  - Cyprinodontiformes/*genetics/*metabolism
MH  - Female
MH  - Fish Proteins/genetics/metabolism
MH  - Gene Expression/*drug effects
MH  - Liver/drug effects
MH  - Male
MH  - Norfloxacin/*toxicity
MH  - RNA, Messenger/genetics/metabolism
MH  - Toxicity Tests, Subchronic
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - Enzyme activity
OT  - Gene expression
OT  - Norfloxacin
OT  - Sub-chronic toxicity
OT  - Swordtail fish (Xiphophorus helleri)
EDAT- 2015/04/22 06:00
MHDA- 2016/09/01 06:00
CRDT- 2015/04/21 06:00
PHST- 2015/03/31 00:00 [accepted]
PHST- 2015/04/21 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/09/01 06:00 [medline]
AID - 10.1007/s10646-015-1457-1 [pii]
AID - 10.1007/s10646-015-1457-1 [doi]
PST - ppublish
SO  - Ecotoxicology. 2015 Oct;24(7-8):1566-73. doi: 10.1007/s10646-015-1457-1. Epub 
      2015 Apr 19.