PMID- 25893639 OWN - NLM STAT- MEDLINE DCOM- 20160223 LR - 20181202 IS - 1432-2072 (Electronic) IS - 0033-3158 (Linking) VI - 232 IP - 15 DP - 2015 Aug TI - Effect of preexposure on methylphenidate-induced taste avoidance and related BDNF/TrkB activity in the insular cortex of the rat. PG - 2837-47 LID - 10.1007/s00213-015-3924-z [doi] AB - RATIONALE: Exogenous brain-derived neurotrophic factor (BDNF) in the insular cortex (IC) is known to influence conditioned taste avoidance (CTA) learning, but little is known of its endogenous role in the phenomenon. Preexposure to many abusable compounds attenuates their ability to induce CTA, thus providing a possible platform from which to further elucidate the endogenous role of IC BDNF in CTA. OBJECTIVES: The role of IC BDNF in CTA learning was examined by assessing the effect of preexposure to methylphenidate (MPH) on MPH-induced CTA, followed by expression between preexposure groups of BDNF in the IC, central nucleus of the amygdala (CeA), and the nucleus accumbens (NAc). METHODS: Following preexposure to MPH (18 mg/kg), CTAs induced by MPH (0, 10, 18, and 32 mg/kg) were assessed in adult male Sprague-Dawley rats (n = 64). In separate groups (n = 31), differences in BDNF and tropomyosin-related kinase receptor-B (TrkB) were assessed using Western blots following similar preexposure and conditioning procedures. RESULTS: Preexposure to MPH significantly blunted MPH-CTA compared to preexposure to vehicle. Unexpectedly, there were no significant effects of MPH on BDNF activity following CTA, but animals preexposed to MPH exhibited decreased activity in the CeA and enhanced activity in the IC and NAc. CONCLUSIONS: Preexposure to MPH attenuates its aversive effects on subsequent presentations, and BDNF's impact on CTA learning may be dependent upon its temporal relation to other CTA-related intracellular cascades. FAU - Wetzell, B Bradley AU - Wetzell BB AD - Psychopharmacology Laboratory, Department of Psychology, American University, Washington, DC, 20016, USA, bradley.wetzell@american.edu. FAU - Muller, Mirabella M AU - Muller MM FAU - Flax, Shaun M AU - Flax SM FAU - King, Heather E AU - King HE FAU - DeCicco-Skinner, Kathleen AU - DeCicco-Skinner K FAU - Riley, Anthony L AU - Riley AL LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150417 PL - Germany TA - Psychopharmacology (Berl) JT - Psychopharmacology JID - 7608025 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Dopamine Uptake Inhibitors) RN - 207ZZ9QZ49 (Methylphenidate) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Amygdala/drug effects/metabolism MH - Animals MH - Avoidance Learning/*drug effects MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cerebral Cortex/*drug effects/metabolism MH - Dopamine Uptake Inhibitors/*pharmacology MH - Male MH - Methylphenidate/*pharmacology MH - Nucleus Accumbens/drug effects/metabolism MH - Phosphorylation MH - Rats MH - Rats, Sprague-Dawley MH - Receptor, trkB/*metabolism MH - Signal Transduction/drug effects MH - Taste/*drug effects MH - Taste Perception/drug effects EDAT- 2015/04/22 06:00 MHDA- 2016/02/26 06:00 CRDT- 2015/04/21 06:00 PHST- 2014/08/21 00:00 [received] PHST- 2015/03/16 00:00 [accepted] PHST- 2015/04/21 06:00 [entrez] PHST- 2015/04/22 06:00 [pubmed] PHST- 2016/02/26 06:00 [medline] AID - 10.1007/s00213-015-3924-z [doi] PST - ppublish SO - Psychopharmacology (Berl). 2015 Aug;232(15):2837-47. doi: 10.1007/s00213-015-3924-z. Epub 2015 Apr 17.