PMID- 25895206 OWN - NLM STAT- MEDLINE DCOM- 20150505 LR - 20161109 IS - 0507-4088 (Print) IS - 0507-4088 (Linking) VI - 59 IP - 5 DP - 2014 TI - [Genetic characterisation of Powassan virus (POWV) isolated from Haemophysalis longicornis ticks in Primorye and two strains of Tick-borne encephalitis virus (TBEV) (Flaviviridae, Flavivirus): Alma-Arasan virus (AAV) isolated from Ixodes persulcatus ticks in Kazakhstan and Malyshevo virus isolated from Aedes vexans nipponii mosquitoes in Khabarovsk kray]. PG - 18-22 AB - The complete genomes of the three tick-borne flaviviruses (genus Flavivirus, fam. Bunyaviridae) were sequenced: Povassan virus (POWV, strain LEIV-3070Prm, isolated from Haemophysalis logicornis in Primorsky Krai, Russia in 1977), Alma-Arasan virus (AAV, strain LEIV-1380Kaz, isolated from Ixodes persulcatus ticks in Kazakhstan in 1977) and Malyshevo virus (isolated from a pool of Aedes vexans nipponii mosquitoes, in the Khabarovsk Krai, Russia in 1978). It is shown that AAV and Malyshevo virus are the strains of Tick-borne encephalitis virus (TBEV) and belong to Sibirian and Far-Eastern genotypes, respectively (GenBank ID: AAV KJ744033; strain Malyshevo KJ744034). Phylogenetically AAV is closest related (94,6% nt and 98,3% aa identity) to TBEV strains, isolated in Sibiria (Vasilchenko, Aino, Chita-653, Irkutsk-12). Malyshevo virus is closest related (96,4% nt and 98,3% nt identity) to strains of TBEV, isolated in Far Eastern part of Russia (1230, Spassk-72, Primorye-89). POWV LEIV-3070Prm has 99.7% identity with the prototype strain POWV LB, isolated in Canada and 99.5% of isolates with Far-Eastern strains of POWV (Spassk-9 and Nadezdinsk-1991). FAU - L'vov, D K AU - L'vov DK FAU - Al'khovskii, S V AU - Al'khovskii SV FAU - Shchelkanov, M Iu AU - Shchelkanov MIu FAU - Deriabin, P G AU - Deriabin PG FAU - Gitel'man, A K AU - Gitel'man AK FAU - Botikov, A G AU - Botikov AG FAU - Aristova, V A AU - Aristova VA LA - rus SI - GENBANK/KJ744033 SI - GENBANK/KJ744034 PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Russia (Federation) TA - Vopr Virusol JT - Voprosy virusologii JID - 0417337 RN - 0 (Viral Proteins) SB - IM MH - Aedes/*virology MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Flaviviridae/classification/*genetics/isolation & purification MH - Flaviviridae Infections/transmission/*veterinary/virology MH - *Genome, Viral MH - High-Throughput Nucleotide Sequencing MH - Humans MH - Kazakhstan MH - Mice MH - Molecular Sequence Data MH - *Phylogeny MH - Sequence Homology, Nucleic Acid MH - Siberia MH - Ticks/*virology MH - Viral Proteins/*genetics EDAT- 2014/01/01 00:00 MHDA- 2015/05/06 06:00 CRDT- 2015/04/22 06:00 PHST- 2015/04/22 06:00 [entrez] PHST- 2014/01/01 00:00 [pubmed] PHST- 2015/05/06 06:00 [medline] PST - ppublish SO - Vopr Virusol. 2014;59(5):18-22.