PMID- 25895441
OWN - NLM
STAT- MEDLINE
DCOM- 20150909
LR  - 20150619
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 145
IP  - 1
DP  - 2015
TI  - Asynchronous Replication in Lymphocytes from Patients with Inflammatory Bowel 
      Disease and Primary Sclerosing Cholangitis.
PG  - 35-41
LID - 10.1159/000381406 [doi]
AB  - Primary sclerosing cholangitis (PSC) and inflammatory bowel disease (IBD) are 
      associated chronic inflammatory diseases with malignant potential. Loss of 
      replication synchrony during the S-phase of the cell cycle has been shown to be 
      linked to several malignant and premalignant states. This study evaluated 
      temporal differences in replication timing between these diseases. The 
      replication pattern of peripheral blood lymphocytes obtained from patients with 
      PSC and IBD and healthy individuals was analyzed by fluorescence in situ 
      hybridization (FISH) in 2 pairs of alleles, in 15qter and 13qter. Asynchrony was 
      determined by the presence of 1 single and 1 set of double dots in the same cell. 
      Samples from subjects with PSC showed significantly greater temporal differences 
      in replication timing, in contrast to the high level of synchrony observed in 
      samples from healthy individuals (p = 0.045). Samples from IBD patients exhibited 
      a nonsignificant increase in replication asynchrony. We believe that these 
      results reflect impairment in the replication control of structural homologous 
      loci in PSC, and that this phenomenon may be correlated with the 
      inflammation-induced malignant potential of this condition.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Laish, Ido
AU  - Laish I
AD  - Institute of Gastroenterology and Hepatology, Meir Medical Center, Kfar Saba, 
      Israel.
FAU - Biron-Shental, Tal
AU  - Biron-Shental T
FAU - Katz, Hila
AU  - Katz H
FAU - Liberman, Meytal
AU  - Liberman M
FAU - Kitay-Cohen, Yona
AU  - Kitay-Cohen Y
FAU - Konikoff, Fred Meir
AU  - Konikoff FM
FAU - Amiel, Aliza
AU  - Amiel A
LA  - eng
PT  - Journal Article
DEP - 20150411
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Cell Division/genetics
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cholangitis, Sclerosing/*genetics
MH  - Colorectal Neoplasms/epidemiology
MH  - *DNA Replication
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Inflammatory Bowel Diseases/*genetics
MH  - Lymphocytes/*pathology
MH  - Male
MH  - Middle Aged
EDAT- 2015/04/22 06:00
MHDA- 2015/09/10 06:00
CRDT- 2015/04/22 06:00
PHST- 2015/02/19 00:00 [accepted]
PHST- 2015/04/22 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2015/09/10 06:00 [medline]
AID - 000381406 [pii]
AID - 10.1159/000381406 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2015;145(1):35-41. doi: 10.1159/000381406. Epub 2015 Apr 
      11.