PMID- 25895812
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 35
IP  - 6
DP  - 2015
TI  - Altered MicroRNA Expression Profiles in Activated Mast Cells Following IgE-FcepsilonRI 
      Cross-Linking with Antigen.
PG  - 2098-110
LID - 10.1159/000374016 [doi]
AB  - BACKGROUND/AIMS: MicroRNAs (miRNAs) are critical regulators of immune responses 
      and immunologic disorders. However, little is known about miRNA expression and 
      function during mast cell differentiation, proliferation and activation. This 
      study aimed to determine the miRNA expression profiles in mast cells stimulated 
      by immunoglobulin E (IgE) and antigen and to analyze the potential functions of 
      specific miRNAs. METHODS: Bone marrow-derived mast cells (BMMCs) generated from 
      differentiated mouse bone marrow cells were untreated (Unstimu) or stimulated 
      with IgE-antigen complexes for 1 h or 6 h (Stimu). The miRNA profiles were 
      evaluated by miRNA microarray. MiRNA target gene prediction and enrichment 
      analyses were performed using bioinformatics. RESULTS: Seven significantly 
      up-regulated and 10 down-regulated miRNAs were identified in the 1 h Stimu group 
      relative to the Unstimu group (fold change>2; P<0.05). Of 8 miRNAs randomly 
      selected from the 17 identified, the expression levels of 6 were confirmed by 
      quantitative real-time PCR (qRT-PCR). The potential target genes of several 
      candidate miRNAs were enriched in FcepsilonRI signaling, response to stimulus and 
      cellular exocytosis. CONCLUSION: The expression of many miRNAs changes following 
      IgE-FcepsilonRI cross-linking in activated mast cells, and these miRNAs probably play 
      key regulatory roles in core signaling pathways and biological behaviors. 
      Evaluating the functions of these characteristic miRNAs will further our 
      understanding of IgE-associated allergic disease pathogenesis and the development 
      of therapeutic strategies.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Teng, Yaoshu
AU  - Teng Y
AD  - Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai, 
      China.
FAU - Zhang, Ruxin
AU  - Zhang R
FAU - Yu, Hongzhi
AU  - Yu H
FAU - Wang, Hong
AU  - Wang H
FAU - Hong, Zhicong
AU  - Hong Z
FAU - Zhuang, Wenjie
AU  - Zhuang W
FAU - Huang, Yu
AU  - Huang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150407
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antigens)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigens/*immunology
MH  - Bone Marrow Cells/immunology
MH  - Cell Differentiation/genetics/immunology
MH  - Down-Regulation/genetics/immunology
MH  - Exocytosis/genetics/immunology
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation/genetics/immunology
MH  - Immunoglobulin E/*immunology
MH  - Male
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*genetics/immunology
MH  - Receptors, IgE/*immunology
MH  - Signal Transduction/genetics/immunology
MH  - Transcriptome/*genetics/immunology
MH  - Up-Regulation/genetics/immunology
EDAT- 2015/04/22 06:00
MHDA- 2016/02/09 06:00
CRDT- 2015/04/22 06:00
PHST- 2015/02/09 00:00 [accepted]
PHST- 2015/04/22 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
AID - 000374016 [pii]
AID - 10.1159/000374016 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2015;35(6):2098-110. doi: 10.1159/000374016. Epub 2015 Apr 
      7.