PMID- 25896131
OWN - NLM
STAT- MEDLINE
DCOM- 20160216
LR  - 20181113
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 405
IP  - 1-2
DP  - 2015 Jul
TI  - Hyperhomocysteinemia regulated SCF expression in cultured cardiomyocytes via 
      modulation of NF-kappaB activities.
PG  - 197-203
LID - 10.1007/s11010-015-2411-5 [doi]
AB  - Hyperhomocysteinemia (HHcy) is an important, independent risk factor for coronary 
      artery disease, especially for the myocardial infarction. Our previous study has 
      shown that myocardial stem cell factor (SCF) mediated cardiac stem cells 
      migration, which was involved in cardiac repair. However, it is not clear 
      regarding the action of HHcy on the expression of SCF in cardiomyocytes. In the 
      present study, cultured neonatal rat cardiomyocytes were treated with 20, 50, or 
      100 muM homocysteine (Hcy) for 5 h. Results showed an significantly increase of 
      SCF expression with 20-50 muM Hcy incubation, which matched with elevated nuclear 
      factor-kappaB (NF-kappaB) activities. Treatment with NF-kappaB inhibitor N-acetylcysteine 
      significantly inhibited the increase of SCF. Nevertheless, 100 muM Hcy markedly 
      decreased the expression of SCF, which was in accordance with the suppression of 
      NF-kappaB activities. The present study indicated that HHcy regulated the expression 
      of SCF in a concentration-dependent manner via modulation of NF-kappaB activities. 
      Thus, HHcy may increase the risk for cardiovascular diseases not only by causing 
      endothelial dysfunction but also by directly exerting detrimental effects on 
      cardiomyocytes.
FAU - Zhao, Xia
AU  - Zhao X
AD  - Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, 1095 Jiefang Da Dao, Wuhan, 430030, China, 
      yespeony@126.com.
FAU - Kuang, Dong
AU  - Kuang D
FAU - Duan, Yuping
AU  - Duan Y
FAU - Xiao, Guixiang
AU  - Xiao G
FAU - Ni, Juan
AU  - Ni J
FAU - Duan, Yaqi
AU  - Duan Y
FAU - Wang, Guoping
AU  - Wang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150421
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (NF-kappa B)
RN  - 0 (Stem Cell Factor)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Animals
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Hyperhomocysteinemia/*metabolism
MH  - Myocytes, Cardiac/drug effects/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cell Factor/*metabolism
EDAT- 2015/04/22 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/04/22 06:00
PHST- 2014/12/26 00:00 [received]
PHST- 2015/04/09 00:00 [accepted]
PHST- 2015/04/22 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - 10.1007/s11010-015-2411-5 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2015 Jul;405(1-2):197-203. doi: 10.1007/s11010-015-2411-5. Epub 
      2015 Apr 21.