PMID- 25896938
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20161125
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1614
DP  - 2015 Jul 21
TI  - Regulation on Beclin-1 expression by mTOR in CoCl2-induced HT22 cell 
      ischemia-reperfusion injury.
PG  - 60-6
LID - S0006-8993(15)00305-4 [pii]
LID - 10.1016/j.brainres.2015.04.016 [doi]
AB  - It has been reported that cerebral ischemia/reperfusion (I/R) injury can activate 
      autophagy. However, the role of autophagy in cerebral I/R injury remains 
      controversy. Two major proteins, mTOR and Beclin-1, govern the formation of 
      autophagosomes to regulate autophagy activity. However, the cross-talking between 
      Beclin-1 and mTOR in cerebral I/R injury remains elusive. In this study, global 
      cerebral I/R injury animal model and focal cerebral I/R injury animal model were 
      induced to test the variation of Beclin-1 level in vivo. To further confirm the 
      variation of Beclin-1 level and investigate the cross-talking between Beclin-1 
      and mammalian target of rapamycin (mTOR) in I/R injury, we used cobalt chloride 
      (CoCl2) to develop an I/R injury cell model in HT22 cell line. Our data showed 
      that the levels of Beclin-1 and phosphorylated mammalian target of rapamycin 
      (p-mTOR) were clearly induced by I/R injury in vitro. And the time course studies 
      suggested that the Beclin-1 and mTOR may have coordinated regulation in ischemia 
      stages but not in reperfusion stages. Moreover, inhibitor of mTOR could prevent 
      Beclin-1 decreasing, but this prevention may play opposite roles in different 
      stages of I/R injury. We conclude that this study represents a major advance in 
      our understanding of the cross-talking of two key proteins, Beclin-1 and mTOR, in 
      autophagy and the role of autophagy in cerebral I/R injury.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Yang, Tao
AU  - Yang T
AD  - Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong 
      University, 277 West Yanta West Road, Xi'an, Shaanxi 710061, PR China; Department 
      of Neurosurgery, Ankang City Central Hospital, Ankang, Shaanxi 725000, PR China.
FAU - Li, Dongbo
AU  - Li D
AD  - Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong 
      University, 277 West Yanta West Road, Xi'an, Shaanxi 710061, PR China; Department 
      of Neurosurgery, Ankang City Central Hospital, Ankang, Shaanxi 725000, PR China.
FAU - Liu, Feng
AU  - Liu F
AD  - Department of Neurosurgery, Ankang City Central Hospital, Ankang, Shaanxi 725000, 
      PR China.
FAU - Qi, Lei
AU  - Qi L
AD  - Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong 
      University, 277 West Yanta West Road, Xi'an, Shaanxi 710061, PR China.
FAU - Yan, Ge
AU  - Yan G
AD  - Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong 
      University, 277 West Yanta West Road, Xi'an, Shaanxi 710061, PR China.
FAU - Wang, Maode
AU  - Wang M
AD  - Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong 
      University, 277 West Yanta West Road, Xi'an, Shaanxi 710061, PR China. Electronic 
      address: maodewang2015@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150418
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Beclin-1)
RN  - 0 (Becn1 protein, rat)
RN  - 3G0H8C9362 (Cobalt)
RN  - EVS87XF13W (cobaltous chloride)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antimutagenic Agents/*pharmacology
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Beclin-1
MH  - Brain Ischemia/pathology
MH  - Cell Line
MH  - Cell Survival
MH  - Cobalt/*pharmacology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Mice
MH  - *Neurons/drug effects/metabolism/pathology
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*chemically induced
MH  - Sirolimus/*toxicity
OTO - NOTNLM
OT  - Autophagy
OT  - Beclin-1
OT  - Ischemia
OT  - Reperfusion
OT  - mTOR
EDAT- 2015/04/22 06:00
MHDA- 2016/02/24 06:00
CRDT- 2015/04/22 06:00
PHST- 2015/01/20 00:00 [received]
PHST- 2015/03/25 00:00 [revised]
PHST- 2015/04/09 00:00 [accepted]
PHST- 2015/04/22 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
AID - S0006-8993(15)00305-4 [pii]
AID - 10.1016/j.brainres.2015.04.016 [doi]
PST - ppublish
SO  - Brain Res. 2015 Jul 21;1614:60-6. doi: 10.1016/j.brainres.2015.04.016. Epub 2015 
      Apr 18.