PMID- 25897683
OWN - NLM
STAT- MEDLINE
DCOM- 20160323
LR  - 20181113
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Print)
IS  - 1096-620X (Linking)
VI  - 18
IP  - 7
DP  - 2015 Jul
TI  - Lonicera japonica THUNB. Extract Inhibits Lipopolysaccharide-Stimulated 
      Inflammatory Responses by Suppressing NF-kappaB Signaling in BV-2 Microglial Cells.
PG  - 762-75
LID - 10.1089/jmf.2014.3341 [doi]
AB  - In the current study, we evaluated the anti-inflammatory effects of Lonicera 
      japonica THUNB. (LJ) and its underlying molecular mechanism in lipopolysaccharide 
      (LPS)-stimulated BV-2 microglial cells. Our results indicated that LJ 
      significantly inhibits LPS-stimulated production of nitric oxide (NO) and 
      prostaglandin E2 (PGE2). In addition, LJ inhibited inducible nitric oxide 
      synthase (iNOS) and cyclooxygenase-2 (COX-2) at both the protein and mRNA levels. 
      In LPS-stimulated BV-2 microglial cells, LJ inhibited proinflammatory cytokines 
      and chemokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), monocyte 
      chemoattractant protein-1 (MCP-1), matrix metalloproteinase-9 (MMP-9) enzymatic 
      activities, and/or mRNA expression, as well as reactive oxygen species (ROS) 
      production. LJ significantly suppressed activation of nuclear factor-kappaB (NF-kappaB) 
      and its translocation from the cytosol to the nucleus and suppressed the 
      DNA-binding activity of NF-kappaB. Furthermore, LJ significantly inhibited 
      phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated 
      kinase 1/2 (ERK 1/2), p38 mitogen-activated protein kinases (MAPKs), 
      phosphatidylinositol 3-kinases (PI3K)/Akt, and Janus kinase 1 (JAK1)/signal 
      transducer and activator of transcription (STAT)1/3. Collectively, our findings 
      indicated that the antineuroinflammatory properties of LJ in LPS-induced BV-2 
      microglial cells is due to downregulation of proinflammatory cytokines and 
      chemokines downstream of inhibition of NF-kappaB activation.
FAU - Kwon, Seung-Hwan
AU  - Kwon SH
AD  - 1 Department of Pharmacology, School of Pharmacy, Sungkyunkwan University , 
      Suwon, Korea.
AD  - 2 Natural Products Research Center, Korea Institute of Science and Technology , 
      Gangneung, Korea.
FAU - Ma, Shi-Xun
AU  - Ma SX
AD  - 1 Department of Pharmacology, School of Pharmacy, Sungkyunkwan University , 
      Suwon, Korea.
FAU - Hong, Sa-Ik
AU  - Hong SI
AD  - 1 Department of Pharmacology, School of Pharmacy, Sungkyunkwan University , 
      Suwon, Korea.
FAU - Lee, Seok-Yong
AU  - Lee SY
AD  - 1 Department of Pharmacology, School of Pharmacy, Sungkyunkwan University , 
      Suwon, Korea.
FAU - Jang, Choon-Gon
AU  - Jang CG
AD  - 1 Department of Pharmacology, School of Pharmacy, Sungkyunkwan University , 
      Suwon, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150421
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (Prostaglandins E)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chemokines/antagonists & inhibitors
MH  - Cyclooxygenase 2/genetics
MH  - Cytokines/antagonists & inhibitors
MH  - Gene Expression/drug effects
MH  - Lipopolysaccharides/*pharmacology
MH  - Lonicera/*chemistry
MH  - Microglia/*metabolism
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Nitric Oxide/antagonists & inhibitors
MH  - Nitric Oxide Synthase Type II/genetics
MH  - Phosphorylation/drug effects
MH  - Plant Extracts/*pharmacology
MH  - Prostaglandins E/antagonists & inhibitors
MH  - RNA, Messenger/analysis
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
PMC - PMC4492671
OTO - NOTNLM
OT  - BV-2 microglial cells
OT  - Inflammatory responses
OT  - Lipopolysaccharide
OT  - Lonicera japonica THUNB
OT  - Nuclear factor-kappaB
EDAT- 2015/04/22 06:00
MHDA- 2016/03/24 06:00
PMCR- 2016/07/01
CRDT- 2015/04/22 06:00
PHST- 2015/04/22 06:00 [entrez]
PHST- 2015/04/22 06:00 [pubmed]
PHST- 2016/03/24 06:00 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 10.1089/jmf.2014.3341 [pii]
AID - 10.1089/jmf.2014.3341 [doi]
PST - ppublish
SO  - J Med Food. 2015 Jul;18(7):762-75. doi: 10.1089/jmf.2014.3341. Epub 2015 Apr 21.