PMID- 25899451
OWN - NLM
STAT- MEDLINE
DCOM- 20160310
LR  - 20220321
IS  - 1432-0428 (Electronic)
IS  - 0012-186X (Linking)
VI  - 58
IP  - 7
DP  - 2015 Jul
TI  - Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and 
      inflammation in mice.
PG  - 1587-600
LID - 10.1007/s00125-015-3583-y [doi]
AB  - AIMS/HYPOTHESIS: Adipose tissue (AT) distribution is a major determinant of 
      mortality and morbidity in obesity. In mice, intra-abdominal transplantation of 
      subcutaneous AT (SAT) protects against glucose intolerance and insulin resistance 
      (IR), but the underlying mechanisms are not well understood. METHODS: We 
      investigated changes in adipokines, tissue-specific glucose uptake, gene 
      expression and systemic inflammation in male C57BL6/J mice implanted 
      intra-abdominally with either inguinal SAT or epididymal visceral AT (VAT) and 
      fed a high-fat diet (HFD) for up to 17 weeks. RESULTS: Glucose tolerance was 
      improved in mice receiving SAT after 6 weeks, and this was not attributable to 
      differences in adiposity, tissue-specific glucose uptake, or plasma leptin or 
      adiponectin concentrations. Instead, SAT transplantation prevented HFD-induced 
      hepatic triacylglycerol accumulation and normalised the expression of hepatic 
      gluconeogenic enzymes. Grafted fat displayed a significant increase in glucose 
      uptake and unexpectedly, an induction of skeletal muscle-specific gene 
      expression. Mice receiving subcutaneous fat also displayed a marked reduction in 
      the plasma concentrations of several proinflammatory cytokines (TNF-alpha, IL-17, 
      IL-12p70, monocyte chemoattractant protein-1 [MCP-1] and macrophage inflammatory 
      protein-1beta [MuIP-1beta]), compared with sham-operated mice. Plasma IL-17 and MIP-1beta 
      concentrations were reduced from as early as 4 weeks after transplantation, and 
      differences in plasma TNF-alpha and IL-17 concentrations predicted glucose tolerance 
      and insulinaemia in the entire cohort of mice (n = 40). In contrast, mice 
      receiving visceral fat transplants were glucose intolerant, with increased 
      hepatic triacylglycerol content and elevated plasma IL-6 concentrations. 
      CONCLUSIONS/INTERPRETATION: Intra-abdominal transplantation of subcutaneous fat 
      reverses HFD-induced glucose intolerance, hepatic triacylglycerol accumulation 
      and systemic inflammation in mice.
FAU - Hocking, Samantha L
AU  - Hocking SL
AD  - Diabetes and Metabolism Division, Garvan Institute of Medical Research, 384 
      Victoria Street, Darlinghurst, 2010, Sydney, NSW, Australia.
FAU - Stewart, Rebecca L
AU  - Stewart RL
FAU - Brandon, Amanda E
AU  - Brandon AE
FAU - Suryana, Eurwin
AU  - Suryana E
FAU - Stuart, Ella
AU  - Stuart E
FAU - Baldwin, Emily M
AU  - Baldwin EM
FAU - Kolumam, Ganesh A
AU  - Kolumam GA
FAU - Modrusan, Zora
AU  - Modrusan Z
FAU - Junutula, Jagath R
AU  - Junutula JR
FAU - Gunton, Jenny E
AU  - Gunton JE
FAU - Medynskyj, Michael
AU  - Medynskyj M
FAU - Blaber, Sinead P
AU  - Blaber SP
FAU - Karsten, Elisabeth
AU  - Karsten E
FAU - Herbert, Benjamin R
AU  - Herbert BR
FAU - James, David E
AU  - James DE
FAU - Cooney, Gregory J
AU  - Cooney GJ
FAU - Swarbrick, Michael M
AU  - Swarbrick MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150422
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
RN  - 0 (Adiponectin)
RN  - 0 (Cytokines)
RN  - 0 (Insulin)
RN  - 0 (Leptin)
RN  - 0 (Triglycerides)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adipocytes/metabolism/ultrastructure
MH  - Adiponectin/blood
MH  - Adiposity
MH  - Animals
MH  - Body Composition
MH  - Cytokines/blood
MH  - Diet, High-Fat/adverse effects
MH  - Eating
MH  - Gluconeogenesis
MH  - Glucose/metabolism
MH  - Glucose Intolerance/*surgery
MH  - Inflammation/*surgery
MH  - Insulin/blood
MH  - Leptin/blood
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Subcutaneous Fat/*transplantation
MH  - Triglycerides/metabolism
EDAT- 2015/04/23 06:00
MHDA- 2016/03/11 06:00
CRDT- 2015/04/23 06:00
PHST- 2014/10/30 00:00 [received]
PHST- 2015/03/13 00:00 [accepted]
PHST- 2015/04/23 06:00 [entrez]
PHST- 2015/04/23 06:00 [pubmed]
PHST- 2016/03/11 06:00 [medline]
AID - 10.1007/s00125-015-3583-y [doi]
PST - ppublish
SO  - Diabetologia. 2015 Jul;58(7):1587-600. doi: 10.1007/s00125-015-3583-y. Epub 2015 
      Apr 22.