PMID- 25899830
OWN - NLM
STAT- MEDLINE
DCOM- 20151013
LR  - 20191023
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 230
IP  - 11
DP  - 2015 Nov
TI  - Myeloid Zinc Finger 1 (MZF-1) Regulates Expression of the CCN2/CTGF and CCN3/NOV 
      Genes in the Hematopoietic Compartment.
PG  - 2634-9
LID - 10.1002/jcp.25021 [doi]
AB  - Connective Tissue Growth Factor (CCN2/CTGF) and Nephroblastoma Overexpressed 
      (CCN3/NOV) execute key functions within the hematopoietic compartment. Both are 
      abundant in the bone marrow stroma, which is a niche for hematopoiesis and 
      supports marrow function. Roles for 1,25-dihydroxyvitamin D3 (calcitriol) and 
      all-trans retinoic acid in the bone marrow have also been elucidated. 
      Interestingly, some of the annotated roles of these vitamins overlap with 
      established functions of CCN2 and CCN3. Yet, no factor has been identified that 
      unifies these observations. In this study, we report the regulation of the CTGF 
      and NOV genes by Myeloid Zinc Finger-1 (MZF-1), a hematopoietic transcription 
      factor. We show the interaction of MZF-1 with the CTGF and NOV promoters in 
      several cell types. Up-regulation of MZF-1 via calcitriol and vitamin A induces 
      expression of CTGF and NOV, implicating a role for these vitamins in the 
      functions of these two genes. Lastly, knockdown of MZF1 reduces levels of CTGF 
      and NOV. Collectively, our results argue that MZF-1 regulates the CTGF and NOV 
      genes in the hematopoietic compartment, and may be involved in their respective 
      functions in the stroma.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Piszczatowski, Richard T
AU  - Piszczatowski RT
AD  - Department of Sciences, John Jay College, The City University of New York, New 
      York.
AD  - Albert Einstein College of Medicine, Yeshiva University, New York, New York.
FAU - Rafferty, Brian J
AU  - Rafferty BJ
AD  - Department of Science, Borough of Manhattan Community College, The City 
      University of New York, New York.
FAU - Rozado, Andre
AU  - Rozado A
AD  - Department of Sciences, John Jay College, The City University of New York, New 
      York.
FAU - Parziale, James V
AU  - Parziale JV
AD  - Department of Sciences, John Jay College, The City University of New York, New 
      York.
FAU - Lents, Nathan H
AU  - Lents NH
AD  - Department of Sciences, John Jay College, The City University of New York, New 
      York.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (CCN2 protein, human)
RN  - 0 (CCN3 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MZF1 protein, human)
RN  - 0 (Nephroblastoma Overexpressed Protein)
RN  - 0 (RNA, Messenger)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - 5688UTC01R (Tretinoin)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Bone Marrow/metabolism
MH  - Calcitriol/metabolism
MH  - Connective Tissue Growth Factor/*biosynthesis/genetics
MH  - Gene Expression Regulation
MH  - Hematopoiesis/*genetics
MH  - Humans
MH  - Kruppel-Like Transcription Factors/*genetics/metabolism
MH  - Nephroblastoma Overexpressed Protein/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Tretinoin/metabolism
EDAT- 2015/04/23 06:00
MHDA- 2015/10/16 06:00
CRDT- 2015/04/23 06:00
PHST- 2015/03/27 00:00 [received]
PHST- 2015/04/15 00:00 [accepted]
PHST- 2015/04/23 06:00 [entrez]
PHST- 2015/04/23 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
AID - 10.1002/jcp.25021 [doi]
PST - ppublish
SO  - J Cell Physiol. 2015 Nov;230(11):2634-9. doi: 10.1002/jcp.25021.