PMID- 25900259
OWN - NLM
STAT- MEDLINE
DCOM- 20170109
LR  - 20181202
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 50
IP  - 6
DP  - 2015 Dec
TI  - Periostin of human periodontal ligament fibroblasts promotes migration of human 
      mesenchymal stem cell through the alphavbeta3 integrin/FAK/PI3K/Akt pathway.
PG  - 855-63
LID - 10.1111/jre.12277 [doi]
AB  - BACKGROUND AND OBJECTIVE: The periodontal ligament (PDL) is characterized by 
      rapid turnover, high remodeling capacity and high inherent regenerative potential 
      compared with other connective tissues. Periostin, which is highly expressed in 
      the fibroblasts in the PDL, has been widely discussed in relation to collagen 
      fibrillogenesis in the PDL. Recently, several reports have indicated periostin in 
      cell migration. The aim of this study was to examine whether human PDL 
      fibroblasts (hPDLFs) with high levels of periostin expression promote the 
      migration of human bone marrow mesenchymal stem cells (hMSCs). MATERIAL AND 
      METHODS: The migration of hMSCs was examined by transwell chamber migration assay 
      under different conditions: medium alone, hPDLFs, human dermal fibroblasts, 
      recombinant periostin, integrin alphavbeta3 blocking antibody (anti-CD51/61 antibody) 
      and inhibitors of FAK (PF431396) and PI3K (LY294002). Phosphorylation of FAK and 
      Akt in hMSCs under stimulation of periostin was examined by western blotting. 
      RESULTS: The migration assay revealed that the number of migrated hMSCs by hPDLFs 
      was significantly larger than those by dermal fibroblasts, periostin small 
      interfering RNA hPDLFs and medium alone. Furthermore, recombinant periostin also 
      strongly induced hMSC migration. The addition of anti-CD51/61 antibody, PF431396 
      and LY294002 caused a significant reduction in the number of migrated hMSCs 
      respectively. The anti-CD51/61 antibody inhibited both FAK and Akt 
      phosphorylations under periostin stimulation. PF431396 inhibited both FAK and Akt 
      phosphorylations. LY294002 inhibited only Akt phosphorylation, and FAK 
      phosphorylation was not influenced under periostin stimulation. CONCLUSION: 
      Periostin expression in hPDLFs promotes the migration of hMSCs through the alphavbeta3 
      integrin/FAK/PI3K/Akt pathway in vitro.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Matsuzawa, M
AU  - Matsuzawa M
AD  - Department of Orthodontics, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
FAU - Arai, C
AU  - Arai C
AD  - Department of Orthodontics, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
FAU - Nomura, Y
AU  - Nomura Y
AD  - Department of Translation Research, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
FAU - Murata, T
AU  - Murata T
AD  - Department of Translation Research, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
FAU - Yamakoshi, Y
AU  - Yamakoshi Y
AD  - Department of Biochemistry and Molecular Biology, Tsurumi University School of 
      Dental Medicine, Tsurumi-ku, Yokohama, Japan.
FAU - Oida, S
AU  - Oida S
AD  - Department of Biochemistry and Molecular Biology, Tsurumi University School of 
      Dental Medicine, Tsurumi-ku, Yokohama, Japan.
FAU - Hanada, N
AU  - Hanada N
AD  - Department of Translation Research, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
FAU - Nakamura, Y
AU  - Nakamura Y
AD  - Department of Orthodontics, Tsurumi University School of Dental Medicine, 
      Tsurumi-ku, Yokohama, Japan.
LA  - eng
SI  - RefSeq/NG_007073
SI  - RefSeq/NM_000212
SI  - RefSeq/NM_002210
SI  - RefSeq/NM_006475
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150420
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Integrin alphaVbeta3)
RN  - 0 (POSTN protein, human)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (PTK2 protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Migration Assays
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/*metabolism
MH  - Focal Adhesion Kinase 1/*metabolism
MH  - Humans
MH  - Integrin alphaVbeta3/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*drug effects/physiology
MH  - Periodontal Ligament/*cytology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Sequence Analysis, DNA
MH  - *Signal Transduction
OTO - NOTNLM
OT  - cell migration
OT  - periodontal ligament
OT  - periostin
OT  - signal transduction
EDAT- 2015/04/23 06:00
MHDA- 2017/01/10 06:00
CRDT- 2015/04/23 06:00
PHST- 2015/03/03 00:00 [accepted]
PHST- 2015/04/23 06:00 [entrez]
PHST- 2015/04/23 06:00 [pubmed]
PHST- 2017/01/10 06:00 [medline]
AID - 10.1111/jre.12277 [doi]
PST - ppublish
SO  - J Periodontal Res. 2015 Dec;50(6):855-63. doi: 10.1111/jre.12277. Epub 2015 Apr 
      20.