PMID- 25901741
OWN - NLM
STAT- MEDLINE
DCOM- 20160308
LR  - 20200930
IS  - 1555-8576 (Electronic)
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 16
IP  - 6
DP  - 2015
TI  - GATA-3 augmentation down-regulates Connexin43 in Helicobacter pylori associated 
      gastric carcinogenesis.
PG  - 987-96
LID - 10.1080/15384047.2015.1030552 [doi]
AB  - Helicobacter pylori (H. pylori) is a risk factor of gastric carcinoma, and 
      inflammation with H.pylori infection has widely been suggested to trigger gastric 
      carcinogenesis through "inflammation-carcinoma chain" (non-atrophic gastritis 
      (NAG) --> chronic atrophic gastritis (CAG) --> intestinal metaplasia (IM) --> dysplasia 
      (DYS) and gastric carcinoma (GC)). Connexin43 (Cx43) is a major constituent of 
      gap junction in normal gastric mucosa (NGM) and it is continuously down-regulated 
      from normal gastric mucosa to precancerous lesions or ultimate gastric carcinoma, 
      which shows novel target against gastric carcinoma by preventing the Cx43 
      decline. Our previous studies demonstrated that H. pylori infection in gastric 
      mucosa down-regulates Cx43 expression, but its mechanism remains unknown. The 
      transcriptional factor, GATA binding protein 3 (GATA-3) is the key to regulate 
      adaptive immune response, which possibly relates to inflammation toward malignant 
      transformation. Here the substantial rising of GATA-3 was screened by 
      transcriptional factor microarray along the developmental stages of H. pylori 
      associated gastric carcinoma. Moreover, the increased GATA-3 and inhibited Cx43 
      were confirmed in clinical specimens, Mongolian gerbils and normal gastric 
      epithelial cell line GES-1 with H. pylori infection. GATA-3 silencing generated 
      the Cx43 restoration both in intermediate differentiation gastric cancer cells 
      BGC-803 and in H. pylori infected GES-1 cells. Dual-luciferase reporter assay 
      further revealed the GATA-3 as one of Cx43 down-regulators by directly binding to 
      its promoters. Together, the incremental GATA-3 is found in H. pylori associated 
      gastric carcinogenesis, which is responsible for Cx43 inhibition as well.
FAU - Liu, Xiaoming
AU  - Liu X
AD  - a Department of Gastroenterology ; Third Xiangya Hospital ; Central South 
      University ; Changsha , PR China.
FAU - Cao, Ke
AU  - Cao K
FAU - Xu, Canxia
AU  - Xu C
FAU - Hu, Tingzi
AU  - Hu T
FAU - Zhou, Li
AU  - Zhou L
FAU - Cao, Dan
AU  - Cao D
FAU - Xiao, Jing
AU  - Xiao J
FAU - Luo, Ling
AU  - Luo L
FAU - Guo, Yinjie
AU  - Guo Y
FAU - Qi, Yong
AU  - Qi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150422
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (Connexin 43)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - *Cell Transformation, Neoplastic
MH  - Connexin 43/*genetics
MH  - Disease Models, Animal
MH  - GATA3 Transcription Factor/*genetics
MH  - Gastric Mucosa/metabolism/microbiology/pathology
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gerbillinae
MH  - Helicobacter Infections/*complications/*microbiology
MH  - *Helicobacter pylori
MH  - Humans
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Stomach Neoplasms/*etiology/pathology
MH  - Transcription Factors/metabolism
PMC - PMC4622629
OTO - NOTNLM
OT  - CAG, chronic atrophic gastritis
OT  - CagA, cytotoxin-associated gene A
OT  - Connexin43
OT  - Cx43, connexin43
OT  - DYS, dysplasia
OT  - GATA-3
OT  - GATA-3, GATA binding protein 3
OT  - GC, gastric carcinoma
OT  - GJ, gap junction
OT  - H. pylori, Helicobacter pylori
OT  - Helicobacter pylori
OT  - IFN-gamma, interferon-gamma
OT  - ILC, innate lymphoid cell
OT  - IM, intestinal metaplasia
OT  - NAG, non-atrophic gastritis
OT  - NGM, normal gastric mucosa.
OT  - PBMC, peripheral blood mononuclear cell
OT  - PBS, phosphate buffered saline
OT  - TF, transcriptional factor
OT  - Th1/Th2 cell, type 1/2 T helper cell
OT  - VacA, vacuolating cytotoxin gene A
OT  - carcinogenesis
OT  - gastric carcinoma
OT  - inflammation-carcinoma chain
OT  - transcription factor
EDAT- 2015/04/23 06:00
MHDA- 2016/03/10 06:00
PMCR- 2016/04/22
CRDT- 2015/04/23 06:00
PHST- 2015/04/23 06:00 [entrez]
PHST- 2015/04/23 06:00 [pubmed]
PHST- 2016/03/10 06:00 [medline]
PHST- 2016/04/22 00:00 [pmc-release]
AID - 1030552 [pii]
AID - 10.1080/15384047.2015.1030552 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2015;16(6):987-96. doi: 10.1080/15384047.2015.1030552. Epub 
      2015 Apr 22.