PMID- 25903459
OWN - NLM
STAT- MEDLINE
DCOM- 20160304
LR  - 20181113
IS  - 2047-783X (Electronic)
IS  - 0949-2321 (Print)
IS  - 0949-2321 (Linking)
VI  - 20
IP  - 1
DP  - 2015 Apr 23
TI  - microRNA-98 mediated microvascular hyperpermeability during burn shock phase via 
      inhibiting FIH-1.
PG  - 51
LID - 10.1186/s40001-015-0141-5 [doi]
LID - 51
AB  - BACKGROUND: microRNA is a small non-coding RNA molecule and functions in RNA 
      silencing and post-transcriptional regulation of gene expression. This study was 
      designed to evaluate the role of miR-98 in the development of microvascular 
      permeability and its molecular pathogenesis. METHODS: Forty-eight healthy adult 
      Wistar rats were divided into the control group (n = 8) and burn group (n = 40) 
      that inflicted with 30% total body surface area third-degree burn. Groups were 
      processed at 2, 4, 8, 12, and 24 h post-burn. Plasma for vascular endothelial 
      cell culture was collected from control and 12 h post-burn rats. Organic 
      microvascular permeability and serum miR-98 level were measured. In vitro, rat 
      aorta endothelial cells were stimulated with burn serum. Level of miR-98 and 
      protein of hypoxia-inducible factor-1 (HIF-1), factor inhibiting HIF-1alpha (FIH-1), 
      and tight junction-associated proteins were determined. RESULTS: Organic 
      microvascular permeability began to rise at 2 h post-burn and maintained the same 
      character throughout the experiment except in lung tissue that was still rising 
      at 12 h; the serum level of miR-98 was elevated (P < 0.05). In vitro, burn serum 
      stimulation increased rat aorta endothelial monolayer cell permeability as well 
      as upregulated miR-98 expression (P < 0.05). As shown in the result of 
      transfection experiment, miR-98 negatively regulated FIH-1 and tight 
      junction-associated protein expression (P < 0.05). CONCLUSIONS: The findings of 
      the present study suggest severe microvascular permeability due to burns; and the 
      underlying mechanism bases on the promotion of miR-98 level to the extent that it 
      activated HIF-1 gene expression, resulting in junction-associated protein 
      deficiency.
FAU - Hu, Delin
AU  - Hu D
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      Hu_del@163.com.
FAU - Yu, Youxin
AU  - Yu Y
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      Yyouxin@yeah.net.
FAU - Wang, Chunhua
AU  - Wang C
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      Chunh_w@126.com.
FAU - Li, Denghui
AU  - Li D
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      Ldengh@126.com.
FAU - Tai, Yuncheng
AU  - Tai Y
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      taiyunc@126.com.
FAU - Fang, Linsen
AU  - Fang L
AD  - Department of Burns, The First Affiliated Hospital of Anhui Medical University, 
      No. 218 Jixi Road, Hefei, Anhui Province, 230022, People's Republic of China. 
      fangls@yeah.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150423
PL  - England
TA  - Eur J Med Res
JT  - European journal of medical research
JID - 9517857
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (MIRN98 microRNA-98, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Tight Junction Proteins)
SB  - IM
MH  - Animals
MH  - Burns/complications/*metabolism
MH  - *Capillary Permeability
MH  - Endothelium, Vascular/metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/*metabolism
MH  - MicroRNAs/*genetics
MH  - Microvessels/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Shock, Traumatic/etiology/*metabolism
MH  - Tight Junction Proteins/genetics/metabolism
PMC - PMC4411771
EDAT- 2015/04/24 06:00
MHDA- 2016/03/05 06:00
PMCR- 2015/04/23
CRDT- 2015/04/24 06:00
PHST- 2015/01/06 00:00 [received]
PHST- 2015/04/13 00:00 [accepted]
PHST- 2015/04/24 06:00 [entrez]
PHST- 2015/04/24 06:00 [pubmed]
PHST- 2016/03/05 06:00 [medline]
PHST- 2015/04/23 00:00 [pmc-release]
AID - 10.1186/s40001-015-0141-5 [pii]
AID - 141 [pii]
AID - 10.1186/s40001-015-0141-5 [doi]
PST - epublish
SO  - Eur J Med Res. 2015 Apr 23;20(1):51. doi: 10.1186/s40001-015-0141-5.