PMID- 25904129
OWN - NLM
STAT- MEDLINE
DCOM- 20160606
LR  - 20150916
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 20
DP  - 2015 Oct
TI  - The role of autophagy in the cytotoxicity induced by recombinant human arginase 
      in laryngeal squamous cell carcinoma.
PG  - 8487-94
LID - 10.1007/s00253-015-6565-6 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC), one of the most common malignant solid 
      tumors in the world, has a high rate of mortality, recurrence, and metastasis. 
      Recombinant human arginase (rhArg) recently has been developed in arginine 
      deprivation therapy for a number of malignant tumors. In the present study, we 
      observed that rhArg triggered significant cytotoxicity in human laryngeal 
      squamous cell carcinoma Tu212 cells. Meanwhile, we observed that rhArg 
      simultaneously activated autophagic flux in Tu212 cells, which was demonstrated 
      by the accumulation of autophagosome and light chain 3 II (LC3-II). And, we 
      explored the role of autophagy in cytotoxicity induced by rhArg in Tu212 cells. 
      Autophagy inhibitors including chloroquine (CQ) and bafilomycin A1 (Baf A1) 
      enhanced cytotoxicity induced by rhArg, implying the protective role of autophagy 
      in rhArg-treated Tu212 cells. Moreover, Akt/mTOR signaling pathway was most 
      possibly to participate in the rhArg-induced autophagy. Meanwhile, rhArg could 
      upregulate the phosphorylation of ERK1/2 in a time-dependent manner. Therefore, 
      all the results illuminated the cytoprotective role of autophagy in the treatment 
      of rhArg in laryngeal squamous carcinoma Tu212 cells and provided a potential 
      approach for LSCC therapy by rhArg combined with autophagy inhibitors.
FAU - Lin, Chen
AU  - Lin C
AD  - Department of Otolaryngology, Changzheng Hospital, Second Military Medical 
      University, Shanghai, 200003, China.
FAU - Wang, Ziyu
AU  - Wang Z
AD  - Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of 
      Education, School of Pharmacy, Fudan University, Shanghai, China.
AD  - Department of Biopharmaceutical Research, Shanghai Institute of Pharmaceutical 
      Industry, Shanghai, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Otolaryngology, Changzheng Hospital, Second Military Medical 
      University, Shanghai, 200003, China.
FAU - He, Yong
AU  - He Y
AD  - Department of Otolaryngology, The Affiliated Hospital of School of Medicine of 
      Ningbo University, Ningbo, Zhejiang Province, China.
FAU - Fan, Jiajun
AU  - Fan J
AD  - Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of 
      Education, School of Pharmacy, Fudan University, Shanghai, China.
FAU - Liu, Zhongyu
AU  - Liu Z
AD  - Department of Otolaryngology, Changzheng Hospital, Second Military Medical 
      University, Shanghai, 200003, China.
FAU - Zhao, Shuwei
AU  - Zhao S
AD  - Department of Otolaryngology, Changzheng Hospital, Second Military Medical 
      University, Shanghai, 200003, China. 13621671816@163.com.
FAU - Ju, Dianwen
AU  - Ju D
AD  - Department of Biosynthesis & Key Lab of Smart Drug Delivery of Ministry of 
      Education, School of Pharmacy, Fudan University, Shanghai, China. 
      dianwenju@fudan.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150423
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Recombinant Proteins)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Arginase/genetics/*toxicity
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/*drug effects
MH  - Epithelial Cells/*drug effects/*physiology
MH  - Humans
MH  - Recombinant Proteins/genetics/toxicity
OTO - NOTNLM
OT  - Autophagy
OT  - Cytotoxicity
OT  - Laryngeal squamous cell carcinoma
OT  - Recombinant human arginase
EDAT- 2015/04/24 06:00
MHDA- 2016/06/09 06:00
CRDT- 2015/04/24 06:00
PHST- 2015/01/10 00:00 [received]
PHST- 2015/03/20 00:00 [accepted]
PHST- 2015/03/20 00:00 [revised]
PHST- 2015/04/24 06:00 [entrez]
PHST- 2015/04/24 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
AID - 10.1007/s00253-015-6565-6 [pii]
AID - 10.1007/s00253-015-6565-6 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Oct;99(20):8487-94. doi: 
      10.1007/s00253-015-6565-6. Epub 2015 Apr 23.