PMID- 25906995 OWN - NLM STAT- MEDLINE DCOM- 20151026 LR - 20181113 IS - 1748-7838 (Electronic) IS - 1001-0602 (Print) IS - 1001-0602 (Linking) VI - 25 IP - 5 DP - 2015 May TI - Deficient plastidic fatty acid synthesis triggers cell death by modulating mitochondrial reactive oxygen species. PG - 621-33 LID - 10.1038/cr.2015.46 [doi] AB - Programmed cell death (PCD) is of fundamental importance to development and defense in animals and plants. In plants, a well-recognized form of PCD is hypersensitive response (HR) triggered by pathogens, which involves the generation of reactive oxygen species (ROS) and other signaling molecules. While the mitochondrion is a master regulator of PCD in animals, the chloroplast is known to regulate PCD in plants. Arabidopsis Mosaic Death 1 (MOD1), an enoyl-acyl carrier protein (ACP) reductase essential for fatty acid biosynthesis in chloroplasts, negatively regulates PCD in Arabidopsis. Here we report that PCD in mod1 results from accumulated ROS and can be suppressed by mutations in mitochondrial complex I components, and that the suppression is confirmed by pharmaceutical inhibition of the complex I-generated ROS. We further show that intact mitochondria are required for full HR and optimum disease resistance to the Pseudomonas syringae bacteria. These findings strongly indicate that the ROS generated in the electron transport chain in mitochondria plays a key role in triggering plant PCD and highlight an important role of the communication between chloroplast and mitochondrion in the control of PCD in plants. FAU - Wu, Jian AU - Wu J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Sun, Yuefeng AU - Sun Y AD - 1] State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] Current address: Department of Pathology and Cell Biology, University of South Florida, Tampa, FL 33612, USA. FAU - Zhao, Yannan AU - Zhao Y AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Zhang, Jian AU - Zhang J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Luo, Lilan AU - Luo L AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Li, Meng AU - Li M AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wang, Jinlong AU - Wang J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Yu, Hong AU - Yu H AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Liu, Guifu AU - Liu G AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Yang, Liusha AU - Yang L AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Xiong, Guosheng AU - Xiong G AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Zhou, Jian-Min AU - Zhou JM AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Zuo, Jianru AU - Zuo J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wang, Yonghong AU - Wang Y AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Li, Jiayang AU - Li J AD - State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150424 PL - England TA - Cell Res JT - Cell research JID - 9425763 RN - 0 (Arabidopsis Proteins) RN - 0 (Reactive Oxygen Species) RN - EC 1.3.1.9 (Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)) RN - EC 1.6.3.- (NADPH Oxidases) SB - IM CIN - Oncotarget. 2015 Jun 10;6(16):13852-3. PMID: 26116839 MH - Arabidopsis/genetics/*metabolism MH - Arabidopsis Proteins/genetics/*metabolism MH - Cell Death/genetics/physiology MH - Chloroplasts/genetics/metabolism MH - Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)/genetics/metabolism MH - Mitochondria/*metabolism MH - NADPH Oxidases/genetics/metabolism MH - Pseudomonas syringae/pathogenicity MH - Reactive Oxygen Species/*metabolism PMC - PMC4423084 EDAT- 2015/04/25 06:00 MHDA- 2015/10/27 06:00 PMCR- 2015/05/01 CRDT- 2015/04/25 06:00 PHST- 2014/10/03 00:00 [received] PHST- 2015/01/06 00:00 [revised] PHST- 2015/02/27 00:00 [accepted] PHST- 2015/04/25 06:00 [entrez] PHST- 2015/04/25 06:00 [pubmed] PHST- 2015/10/27 06:00 [medline] PHST- 2015/05/01 00:00 [pmc-release] AID - cr201546 [pii] AID - 10.1038/cr.2015.46 [doi] PST - ppublish SO - Cell Res. 2015 May;25(5):621-33. doi: 10.1038/cr.2015.46. Epub 2015 Apr 24.