PMID- 25907051 OWN - NLM STAT- MEDLINE DCOM- 20160112 LR - 20150424 IS - 1878-5905 (Electronic) IS - 0142-9612 (Linking) VI - 54 DP - 2015 Jun TI - Allogenic dendritic cell and tumor cell fused vaccine for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer. PG - 177-87 LID - S0142-9612(15)00302-6 [pii] LID - 10.1016/j.biomaterials.2015.03.024 [doi] AB - Dendritic cells (DCs) have displayed the promising potential in cancer immunity. How to enhance DCs immunotherapeutic effect in cancer targeted immunotherapy and prevention is still a great challenge. Herein, we report for the first time the allogenic DCs and tumor cell fused vaccine combined with cytokine induced killing cells (CIKs) for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer (GC). The fused vaccine was prepared by PEG mediated fusion between mature DCs and inactive gastric cancer MGC803 cells. The immunotherapeutic and prophylactic potential of the fused cells (FCs) were evaluated in tumor-bearing, post-surgery and tumor free mice models. The migration and homing process of near infrared region quantum dots (NIR-QDs) labeled FCs were investigated by real-time animal imaging system. Results showed that the FCs and FC + CIKs could trigger the tumor-specific CTLs against GC cells, target the tumor tissue initiatively and enhance the prophylactic effects, suppress the tumor growth remarkably in vivo. The potential mechanism is also investigated. In conclusion, allogenic DCs and tumor cell fused vaccine can be used for targeted imaging and enhanced immunotherapeutic efficacy of GC, and the FC + CIKs strategy own great potential in clinical applications such as early therapy and prevention of tumor-metastasis and relapse in near future. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Li, Chao AU - Li C AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Liang, Shujing AU - Liang S AD - Rui-Jin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China. FAU - Zhang, Chunlei AU - Zhang C AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Liu, Yanlei AU - Liu Y AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Yang, Meng AU - Yang M AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Zhang, Jingpu AU - Zhang J AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Zhi, Xiao AU - Zhi X AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Pan, Fei AU - Pan F AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. FAU - Cui, Daxiang AU - Cui D AD - Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Department of Instrument Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China; National Center for Translational Medicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, PR China. Electronic address: dxcui@sjtu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150409 PL - Netherlands TA - Biomaterials JT - Biomaterials JID - 8100316 RN - 0 (Cancer Vaccines) SB - IM MH - Animals MH - Cancer Vaccines/immunology/*therapeutic use MH - Cell Line, Tumor MH - Combined Modality Therapy/methods MH - Cytokine-Induced Killer Cells/immunology/*transplantation MH - Dendritic Cells/immunology/pathology/*transplantation MH - Mice MH - Mice, Inbred C57BL MH - Microscopy, Fluorescence/methods MH - *Quantum Dots MH - Stomach Neoplasms/immunology/*pathology/*therapy MH - Treatment Outcome OTO - NOTNLM OT - Fusion cell OT - Gastric cancer OT - Immunotherapy OT - Targeted imaging OT - Vaccine EDAT- 2015/04/25 06:00 MHDA- 2016/01/13 06:00 CRDT- 2015/04/25 06:00 PHST- 2014/12/01 00:00 [received] PHST- 2015/03/13 00:00 [revised] PHST- 2015/03/15 00:00 [accepted] PHST- 2015/04/25 06:00 [entrez] PHST- 2015/04/25 06:00 [pubmed] PHST- 2016/01/13 06:00 [medline] AID - S0142-9612(15)00302-6 [pii] AID - 10.1016/j.biomaterials.2015.03.024 [doi] PST - ppublish SO - Biomaterials. 2015 Jun;54:177-87. doi: 10.1016/j.biomaterials.2015.03.024. Epub 2015 Apr 9.