PMID- 25907938
OWN - NLM
STAT- MEDLINE
DCOM- 20150925
LR  - 20231213
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 35
IP  - 4
DP  - 2015 Apr
TI  - [Ursolic acid inhibits corneal graft rejection following orthotopic allograft 
      transplantation in rats].
PG  - 530-5
AB  - OBJECTIVE: To investigate the effects of ursolic acid on corneal graft rejection 
      in a rat model of othotopic corneal allograft transplantation. METHODS: 
      Forty-eight recipient Wistar rats were divided into normal control group with 
      saline treatment (group A), autograft group with saline treatment (group B), SD 
      rat allograft group with saline treatment (group C), and SD rat allograft group 
      with intraperitoneal ursolic acid (UA) treatment group (group D). The rats 
      received saline or UC (20 mg.kg(-1).d(-1)) treatment for 12 days following 
      othotopic graft transplantation. The grafts were evaluated using the Larkin 
      corneal rejection rating system, and the graft survival was assessed with 
      Kaplan-Meier analysis. On day 14, the grafts were harvested for histological 
      examination, Western blotting, and assessment of expressions of interlukin-2 
      (IL-2), interferon-gamma (IFN-gamma), nuclear transcription factor-kappaB (NF-kappaB) p65, 
      vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 
      (ICAM-1). RESULTS: The allograft survival was significantly longer in group D 
      than in group C (29.12+/-9.58 vs 9.67+/-2.16 days, P<0.05). UC treatment obviously 
      reduced the expression levels of IL-2, IFN-gamma, NF-kappaBp65, ICAM-1 and VEGF and 
      increased inhibitory kappa B alpha (IkappaB-alpha) expression in the grafts, where no 
      obvious inflammatory cell infiltration or corneal neovascularization was found. 
      CONCLUSION: As a NF-kappaB inhibitor, ursolic acid can prevent corneal 
      neovascularization and corneal allograft rejection to promote graft survival in 
      rats following orthotopic corneal allograft transplantation.
FAU - Wang, Bo
AU  - Wang B
AD  - Department of Ophthalmology, Nanfang Hospital, Southern Medical University, 
      Guangzhou 510515, China. E-mail: Annawb@163.com.
FAU - Wu, Jing
AU  - Wu J
FAU - Ma, Ming
AU  - Ma M
FAU - Li, Ping-Ping
AU  - Li PP
FAU - Yu, Jian
AU  - Yu J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Nfkbia protein, rat)
RN  - 0 (Rela protein, rat)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Triterpenes)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cornea/metabolism
MH  - Corneal Neovascularization/*prevention & control
MH  - *Corneal Transplantation
MH  - Graft Rejection/*prevention & control
MH  - Graft Survival/*drug effects
MH  - I-kappa B Proteins/metabolism
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interferon-gamma/metabolism
MH  - Kaplan-Meier Estimate
MH  - NF-KappaB Inhibitor alpha
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Transcription Factor RelA/metabolism
MH  - Transplantation, Homologous
MH  - Triterpenes/*pharmacology
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Ursolic Acid
EDAT- 2015/04/25 06:00
MHDA- 2015/09/26 06:00
CRDT- 2015/04/25 06:00
PHST- 2015/04/25 06:00 [entrez]
PHST- 2015/04/25 06:00 [pubmed]
PHST- 2015/09/26 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2015 Apr;35(4):530-5.