PMID- 25911453
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150427
LR  - 20181113
IS  - 2051-817X (Print)
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 3
IP  - 4
DP  - 2015 Apr
TI  - Pericyte NF-kappaB activation enhances endothelial cell proliferation and 
      proangiogenic cytokine secretion in vitro.
LID - 10.14814/phy2.12309 [doi]
LID - e12309
AB  - Pericytes are skeletal muscle resident, multipotent stem cells that are localized 
      to the microvasculature. In vivo, studies have shown that they respond to damage 
      through activation of nuclear-factor kappa-B (NF-kappaB), but the downstream effects 
      of NF-kappaB activation on endothelial cell proliferation and cell-cell signaling 
      during repair remain unknown. The purpose of this study was to examine pericyte 
      NF-kappaB activation in a model of skeletal muscle damage; and use genetic 
      manipulation to study the effects of changes in pericyte NF-kappaB activation on 
      endothelial cell proliferation and cytokine secretion. We utilized scratch injury 
      to C2C12 cells in coculture with human primary pericytes to assess NF-kappaB 
      activation and monocyte chemoattractant protein-1 (MCP-1) secretion from 
      pericytes and C2C12 cells. We also cocultured endothelial cells with pericytes 
      that expressed genetically altered NF-kappaB activation levels, and then quantified 
      endothelial cell proliferation and screened the conditioned media for secreted 
      cytokines. Pericytes trended toward greater NF-kappaB activation in injured compared 
      to control cocultures (P = 0.085) and in comparison to C2C12 cells (P = 0.079). 
      Second, increased NF-kappaB activation in pericytes enhanced the proliferation of 
      cocultured endothelial cells (1.3-fold, P = 0.002). Finally, we identified 
      inflammatory signaling molecules, including MCP-1 and interleukin 8 (IL-8) that 
      may mediate the crosstalk between pericytes and endothelial cells. The results of 
      this study show that pericyte NF-kappaB activation may be an important mechanism in 
      skeletal muscle repair with implications for the development of therapies for 
      musculoskeletal and vascular diseases, including peripheral artery disease.
CI  - (c) 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on 
      behalf of the American Physiological Society and The Physiological Society.
FAU - LaBarbera, Katherine E
AU  - LaBarbera KE
AD  - Department of Kinesiology, University of Massachusetts Amherst, Amherst, 
      Massachusetts klabarbe@kin.umass.edu.
FAU - Hyldahl, Robert D
AU  - Hyldahl RD
AD  - Department of Exercise Sciences, Brigham Young University, Provo, Utah.
FAU - O'Fallon, Kevin S
AU  - O'Fallon KS
AD  - Department of Kinesiology, University of Massachusetts Amherst, Amherst, 
      Massachusetts.
FAU - Clarkson, Priscilla M
AU  - Clarkson PM
AD  - Department of Kinesiology, University of Massachusetts Amherst, Amherst, 
      Massachusetts.
FAU - Witkowski, Sarah
AU  - Witkowski S
AD  - Department of Kinesiology, University of Massachusetts Amherst, Amherst, 
      Massachusetts.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
PMC - PMC4425949
OTO - NOTNLM
OT  - Cytokine signaling
OT  - NF-kappaB
OT  - microvasculature
OT  - pericyte
EDAT- 2015/04/26 06:00
MHDA- 2015/04/26 06:01
PMCR- 2015/04/27
CRDT- 2015/04/26 06:00
PHST- 2015/04/26 06:00 [entrez]
PHST- 2015/04/26 06:00 [pubmed]
PHST- 2015/04/26 06:01 [medline]
PHST- 2015/04/27 00:00 [pmc-release]
AID - 3/4/e12309 [pii]
AID - 10.14814/phy2.12309 [doi]
PST - ppublish
SO  - Physiol Rep. 2015 Apr;3(4):e12309. doi: 10.14814/phy2.12309.